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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Jan;171(1):538–546. doi: 10.1128/jb.171.1.538-546.1989

Gene replacement and retrieval with recombinant M13mp bacteriophages.

P Blum 1, D Holzschu 1, H S Kwan 1, D Riggs 1, S Artz 1
PMCID: PMC209619  PMID: 2644206

Abstract

We have developed an allele exchange system for shuttling sequences of DNA to and from their original chromosomal loci. Cloned segments of the histidine operon of Salmonella typhimurium and the lactose operon of Escherichia coli served as target sequences and were used to develop the system. Replacement and retrieval of target sequences used the phage M13mp vectors and proceeded through an M13 lysogen intermediate. The intermediates and products of allele exchange were characterized by genetic and hybridization analyses. Several unique properties of M13 lysogens were exploited to devise positive selections to detect integration and excision. These positive selections were used to manipulate phenotypically silent alleles.

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Selected References

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