Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Jan;171(1):581–584. doi: 10.1128/jb.171.1.581-584.1989

Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.

S Sela 1, D Yogev 1, S Razin 1, H Bercovier 1
PMCID: PMC209627  PMID: 2492503

Abstract

The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.

Full text

PDF
581

Images in this article

582Image
on p.582
582Image
on p.582

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An G., Friesen J. D. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene. 1980 Dec;12(1-2):33–39. doi: 10.1016/0378-1119(80)90013-x. [DOI] [PubMed] [Google Scholar]
  2. Bercovier H., Kafri O., Sela S. Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys Res Commun. 1986 May 14;136(3):1136–1141. doi: 10.1016/0006-291x(86)90452-3. [DOI] [PubMed] [Google Scholar]
  3. Filer D., Dhar R., Furano A. V. The conservation of DNA sequences over very long periods of evolutionary time. Evidence against intergeneric chromosomal transfer as an explanation for the presence of Escherichia coli tuf gene sequences in taxonomically-unrelated prokaryotes. Eur J Biochem. 1981 Nov;120(1):69–77. doi: 10.1111/j.1432-1033.1981.tb05671.x. [DOI] [PubMed] [Google Scholar]
  4. Filer D., Furano A. V. Duplication of the tuf gene, which encodes peptide chain elongation factor Tu, is widespread in Gram-negative bacteria. J Bacteriol. 1981 Dec;148(3):1006–1011. doi: 10.1128/jb.148.3.1006-1011.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Filer D., Furano A. V. Portions of the gene encoding elongation factor Tu are highly conserved in prokaryotes. J Biol Chem. 1980 Jan 25;255(2):728–734. [PubMed] [Google Scholar]
  6. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  7. Furano A. V. Direct demonstration of duplicate tuf genes in enteric bacteria. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3104–3108. doi: 10.1073/pnas.75.7.3104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hori H., Osawa S. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci U S A. 1979 Jan;76(1):381–385. doi: 10.1073/pnas.76.1.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jaskunas S. R., Lindahl L., Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. doi: 10.1038/257458a0. [DOI] [PubMed] [Google Scholar]
  10. Johnson J. L., Francis B. S. Taxonomy of the Clostridia: ribosomal ribonucleic acid homologies among the species. J Gen Microbiol. 1975 Jun;88(2):229–244. doi: 10.1099/00221287-88-2-229. [DOI] [PubMed] [Google Scholar]
  11. Ohama T., Yamao F., Muto A., Osawa S. Organization and codon usage of the streptomycin operon in Micrococcus luteus, a bacterium with a high genomic G + C content. J Bacteriol. 1987 Oct;169(10):4770–4777. doi: 10.1128/jb.169.10.4770-4777.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Park Y. H., Hori H., Suzuki K., Osawa S., Komagata K. Phylogenetic analysis of the coryneform bacteria by 5S rRNA sequences. J Bacteriol. 1987 May;169(5):1801–1806. doi: 10.1128/jb.169.5.1801-1806.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Riley M., Anilionis A. Evolution of the bacterial genome. Annu Rev Microbiol. 1978;32:519–560. doi: 10.1146/annurev.mi.32.100178.002511. [DOI] [PubMed] [Google Scholar]
  14. Rogers M. J., Simmons J., Walker R. T., Weisburg W. G., Woese C. R., Tanner R. S., Robinson I. M., Stahl D. A., Olsen G., Leach R. H. Construction of the mycoplasma evolutionary tree from 5S rRNA sequence data. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1160–1164. doi: 10.1073/pnas.82.4.1160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shibuya M., Nashimoto H., Kaziro Y. Cloning of an EcoRI fragment carrying E. coli tufA gene. Mol Gen Genet. 1979 Feb 26;170(2):231–234. doi: 10.1007/BF00337801. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Sparrow A. H., Nauman A. F. Evolution of genome size by DNA doublings. Science. 1976 May 7;192(4239):524–529. doi: 10.1126/science.1257789. [DOI] [PubMed] [Google Scholar]
  18. Tiboni O., Di Pasquale G., Ciferri O. Two tuf genes in the cyanobacterium Spirulina platensis. J Bacteriol. 1984 Jul;159(1):407–409. doi: 10.1128/jb.159.1.407-409.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woese C. R., Maniloff J., Zablen L. B. Phylogenetic analysis of the mycoplasmas. Proc Natl Acad Sci U S A. 1980 Jan;77(1):494–498. doi: 10.1073/pnas.77.1.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Woese C. R., Stackebrandt E., Ludwig W. What are mycoplasmas: the relationship of tempo and mode in bacterial evolution. J Mol Evol. 1984;21(4):305–316. doi: 10.1007/BF02115648. [DOI] [PubMed] [Google Scholar]
  22. Woese C. R., Stackebrandt E., Macke T. J., Fox G. E. A phylogenetic definition of the major eubacterial taxa. Syst Appl Microbiol. 1985;6:143–151. doi: 10.1016/s0723-2020(85)80047-3. [DOI] [PubMed] [Google Scholar]
  23. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]
  24. van der Meide P. H., Vijgenboom E., Dicke M., Bosch L. Regulation of the expression of tufA and tufB, the two genes coding for the elongation factor EF-Tu in Escherichia coli. FEBS Lett. 1982 Mar 22;139(2):325–330. doi: 10.1016/0014-5793(82)80881-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES