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. 1989 Feb;171(2):947–951. doi: 10.1128/jb.171.2.947-951.1989

Insertion proQ220::Tn5 alters regulation of proline porter II, a transporter of proline and glycine betaine in Escherichia coli.

J L Milner 1, J M Wood 1
PMCID: PMC209686  PMID: 2536686

Abstract

Mutation pro-220::Tn5, which increases the resistance of Escherichia coli to 3,4-dehydroproline (M. E. Stalmach, S. Grothe, and J. M. Wood, J. Bacteriol. 156:481-486, 1983), is not linked to putP, proP, or proU. It was located at 40.4 min on the E. coli chromosomal linkage map, by conjugational and transductional mapping, and is now denoted proQ220::Tn5. Proline porter II was not detectable when proQ220::Tn5 proP+ bacteria were cultivated under optimal conditions or with nutritional stress (amino acid limitation). Toxic proline analog sensitivity and proline porter II activity were partially restored to proQ220::Tn5 proP+ bacteria, but not to a proQ220::Tn5 proP219 strain, by a hyperosmotic shift and by growth under osmotic stress. Elevated expression of a proP::lacZ gene fusion, for bacteria grown under osmotic stress, was not influenced by the proQ220::Tn5 insertion. We propose that the proQ locus encodes a positive regulatory element which elevates proline porter II activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. R., Menzel R., Wood J. M. Biochemistry and regulation of a second L-proline transport system in Salmonella typhimurium. J Bacteriol. 1980 Mar;141(3):1071–1076. doi: 10.1128/jb.141.3.1071-1076.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barron A., Jung J. U., Villarejo M. Purification and characterization of a glycine betaine binding protein from Escherichia coli. J Biol Chem. 1987 Aug 25;262(24):11841–11846. [PubMed] [Google Scholar]
  3. Barron A., May G., Bremer E., Villarejo M. Regulation of envelope protein composition during adaptation to osmotic stress in Escherichia coli. J Bacteriol. 1986 Aug;167(2):433–438. doi: 10.1128/jb.167.2.433-438.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biek D., Roth J. R. Regulation of Tn5 transposition in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6047–6051. doi: 10.1073/pnas.77.10.6047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cairney J., Booth I. R., Higgins C. F. Osmoregulation of gene expression in Salmonella typhimurium: proU encodes an osmotically induced betaine transport system. J Bacteriol. 1985 Dec;164(3):1224–1232. doi: 10.1128/jb.164.3.1224-1232.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cairney J., Booth I. R., Higgins C. F. Salmonella typhimurium proP gene encodes a transport system for the osmoprotectant betaine. J Bacteriol. 1985 Dec;164(3):1218–1223. doi: 10.1128/jb.164.3.1218-1223.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  8. Csonka L. N. A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength. J Bacteriol. 1982 Sep;151(3):1433–1443. doi: 10.1128/jb.151.3.1433-1443.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dunlap V. J., Csonka L. N. Osmotic regulation of L-proline transport in Salmonella typhimurium. J Bacteriol. 1985 Jul;163(1):296–304. doi: 10.1128/jb.163.1.296-304.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gowrishankar J. Identification of osmoresponsive genes in Escherichia coli: evidence for participation of potassium and proline transport systems in osmoregulation. J Bacteriol. 1985 Oct;164(1):434–445. doi: 10.1128/jb.164.1.434-445.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gowrishankar J., Jayashree P., Rajkumari K. Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotolerance. J Bacteriol. 1986 Dec;168(3):1197–1204. doi: 10.1128/jb.168.3.1197-1204.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gowrishankar J. proP-mediated proline transport also plays a role in Escherichia coli osmoregulation. J Bacteriol. 1986 Apr;166(1):331–333. doi: 10.1128/jb.166.1.331-333.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grothe S., Krogsrud R. L., McClellan D. J., Milner J. L., Wood J. M. Proline transport and osmotic stress response in Escherichia coli K-12. J Bacteriol. 1986 Apr;166(1):253–259. doi: 10.1128/jb.166.1.253-259.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Higgins C. F., Sutherland L., Cairney J., Booth I. R. The osmotically regulated proU locus of Salmonella typhimurium encodes a periplasmic betaine-binding protein. J Gen Microbiol. 1987 Feb;133(2):305–310. doi: 10.1099/00221287-133-2-305. [DOI] [PubMed] [Google Scholar]
  15. May G., Faatz E., Villarejo M., Bremer E. Binding protein dependent transport of glycine betaine and its osmotic regulation in Escherichia coli K12. Mol Gen Genet. 1986 Nov;205(2):225–233. doi: 10.1007/BF00430432. [DOI] [PubMed] [Google Scholar]
  16. McEwen J., Silverman P. Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J Bacteriol. 1980 Oct;144(1):60–67. doi: 10.1128/jb.144.1.60-67.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Milner J. L., Grothe S., Wood J. M. Proline porter II is activated by a hyperosmotic shift in both whole cells and membrane vesicles of Escherichia coli K12. J Biol Chem. 1988 Oct 15;263(29):14900–14905. [PubMed] [Google Scholar]
  18. Milner J. L., McClellan D. J., Wood J. M. Factors reducing and promoting the effectiveness of proline as an osmoprotectant in Escherichia coli K12. J Gen Microbiol. 1987 Jul;133(7):1851–1860. doi: 10.1099/00221287-133-7-1851. [DOI] [PubMed] [Google Scholar]
  19. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perroud B., Le Rudulier D. Glycine betaine transport in Escherichia coli: osmotic modulation. J Bacteriol. 1985 Jan;161(1):393–401. doi: 10.1128/jb.161.1.393-401.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  22. Ronson C. W., Nixon B. T., Ausubel F. M. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli. Cell. 1987 Jun 5;49(5):579–581. doi: 10.1016/0092-8674(87)90530-7. [DOI] [PubMed] [Google Scholar]
  23. Stalmach M. E., Grothe S., Wood J. M. Two proline porters in Escherichia coli K-12. J Bacteriol. 1983 Nov;156(2):481–486. doi: 10.1128/jb.156.2.481-486.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sutherland L., Cairney J., Elmore M. J., Booth I. R., Higgins C. F. Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium. J Bacteriol. 1986 Nov;168(2):805–814. doi: 10.1128/jb.168.2.805-814.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wood J. M. Genetics of L-proline utilization in Escherichia coli. J Bacteriol. 1981 Jun;146(3):895–901. doi: 10.1128/jb.146.3.895-901.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wood J. M., Zadworny D. Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can J Biochem. 1980 Oct;58(10):787–796. doi: 10.1139/o80-110. [DOI] [PubMed] [Google Scholar]
  27. Wood J. M., Zadworny D. Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12. Can J Biochem. 1979 Oct;57(10):1191–1199. doi: 10.1139/o79-155. [DOI] [PubMed] [Google Scholar]
  28. Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]

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