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. 1989 Feb;171(2):1188–1191. doi: 10.1128/jb.171.2.1188-1191.1989

Point mutations in the regulatory region of the ilvGMEDA operon of Escherichia coli K-12.

R P Lawther 1
PMCID: PMC209719  PMID: 2644227

Abstract

The ilvGMEDA operon of Escherichia coli K-12 is preceded by a regulatory region containing a promoter, a leader, and an attenuator. This region has been extensively characterized biochemically. In this note point mutations of the regulatory region are reported. The effect of these mutations on expression from the ilv regulatory region supports the previous biochemical analysis.

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Selected References

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  1. Bennett D. C., Umbarger H. E. Isolation and analysis of two Escherichia coli K-12 ilv attenuator deletion mutants with high-level constitutive expression of an ilv-lac fusion operon. J Bacteriol. 1984 Mar;157(3):839–845. doi: 10.1128/jb.157.3.839-845.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Busby S., Irani M., Crombrugghe B. Isolation of mutant promoters in the Escherichia coli galactose operon using local mutagenesis on cloned DNA fragments. J Mol Biol. 1982 Jan 15;154(2):197–209. doi: 10.1016/0022-2836(82)90060-2. [DOI] [PubMed] [Google Scholar]
  3. Carter P. W., Weiss D. L., Weith H. L., Calvo J. M. Mutations that convert the four leucine codons of the Salmonella typhimurium leu leader to four threonine codons. J Bacteriol. 1985 Jun;162(3):943–949. doi: 10.1128/jb.162.3.943-949.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cortese R., Landsberg R., Haar R. A., Umbarger H. E., Ames B. N. Pleiotropy of hisT mutants blocked in pseudouridine synthesis in tRNA: leucine and isoleucine-valine operons. Proc Natl Acad Sci U S A. 1974 May;71(5):1857–1861. doi: 10.1073/pnas.71.5.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harms E., Hsu J. H., Subrahmanyam C. S., Umbarger H. E. Comparison of the regulatory regions of ilvGEDA operons from several enteric organisms. J Bacteriol. 1985 Oct;164(1):207–216. doi: 10.1128/jb.164.1.207-216.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harms E., Umbarger H. E. Role of codon choice in the leader region of the ilvGMEDA operon of Serratia marcescens. J Bacteriol. 1987 Dec;169(12):5668–5677. doi: 10.1128/jb.169.12.5668-5677.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hsu J. H., Harms E., Umbarger H. E. Leucine regulation of the ilvGEDA operon of Serratia marcescens by attenuation is modulated by a single leucine codon. J Bacteriol. 1985 Oct;164(1):217–222. doi: 10.1128/jb.164.1.217-222.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnston H. M., Roth J. R. DNA sequence changes of mutations altering attenuation control of the histidine operon of Salmonella typhimurium. J Mol Biol. 1981 Feb 5;145(4):735–756. doi: 10.1016/0022-2836(81)90312-0. [DOI] [PubMed] [Google Scholar]
  10. Lawther R. P., Calhoun D. H., Adams C. W., Hauser C. A., Gray J., Hatfield G. W. Molecular basis of valine resistance in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1981 Feb;78(2):922–925. doi: 10.1073/pnas.78.2.922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lawther R. P., Hatfield G. W. Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1862–1866. doi: 10.1073/pnas.77.4.1862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lawther R. P., Hatfield W. Biochemical characterization of an Escherichia coli hisT strain. J Bacteriol. 1977 Apr;130(1):552–557. doi: 10.1128/jb.130.1.552-557.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lawther R. P., Wek R. C., Lopes J. M., Pereira R., Taillon B. E., Hatfield G. W. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987 Mar 11;15(5):2137–2155. doi: 10.1093/nar/15.5.2137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lynn S. P., Bauer C. E., Chapman K., Gardner J. F. Identification and characterization of mutants affecting transcription termination at the threonine operon attenuator. J Mol Biol. 1985 Jun 25;183(4):529–541. doi: 10.1016/0022-2836(85)90169-x. [DOI] [PubMed] [Google Scholar]
  15. Lynn S. P., Burton W. S., Donohue T. J., Gould R. M., Gumport R. I., Gardner J. F. Specificity of the attenuation response of the threonine operon of Escherichia coli is determined by the threonine and isoleucine codons in the leader transcript. J Mol Biol. 1987 Mar 5;194(1):59–69. doi: 10.1016/0022-2836(87)90715-7. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  17. Nargang F. E., Subrahmanyam C. S., Umbarger H. E. Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1823–1827. doi: 10.1073/pnas.77.4.1823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ortuno M. J., Lawther R. P. Effect of the deletion of upstream DNA sequences on expression from the ilvGp2 promoter of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987 Feb 25;15(4):1521–1542. doi: 10.1093/nar/15.4.1521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Piette J., Cunin R., Boyen A., Charlier D., Crabeel M., Van Vliet F., Glansdorff N., Squires C., Squires C. L. The regulatory region of the divergent argECBH operon in Escherichia coli K-12. Nucleic Acids Res. 1982 Dec 20;10(24):8031–8048. doi: 10.1093/nar/10.24.8031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosen E. D., Hartley J. L., Matz K., Nichols B. P., Young K. M., Donelson J. E., Gussin G. N. DNA sequence analysis of prm-mutations of coliphage lambda. Gene. 1980 Nov;11(3-4):197–205. doi: 10.1016/0378-1119(80)90060-8. [DOI] [PubMed] [Google Scholar]
  21. Stauffer G. V., Zurawski G., Yanofsky C. Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4833–4837. doi: 10.1073/pnas.75.10.4833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taillon M. P., Gotto D. A., Lawther R. P. The DNA sequence of the promoter-attenuator of the ilvGEDA operon of Salmonella typhimurium. Nucleic Acids Res. 1981 Jul 24;9(14):3419–3432. doi: 10.1093/nar/9.14.3419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zoller M. J., Smith M. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 1983;100:468–500. doi: 10.1016/0076-6879(83)00074-9. [DOI] [PubMed] [Google Scholar]
  24. Zurawski G., Elseviers D., Stauffer G. V., Yanofsky C. Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5988–5992. doi: 10.1073/pnas.75.12.5988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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