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. 1989 Mar;171(3):1569–1573. doi: 10.1128/jb.171.3.1569-1573.1989

Construction and shuttling of novel bifunctional vectors for Streptomyces spp. and Escherichia coli.

K Neesen 1, G Volckaert 1
PMCID: PMC209782  PMID: 2646288

Abstract

Shuttle vectors for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of an artificial multicopy E. coli replicon and DNA fragments of pIJ702. Stable transfer to Streptomyces lividans was obtained. Marked differences in transformation efficiency were observed when plasmid DNA isolated from E. coli GM119 was used instead of that from strain HB101.

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Selected References

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