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. 1989 Apr;171(4):1904–1914. doi: 10.1128/jb.171.4.1904-1914.1989

Nucleotide sequences required for Tn3 transposition immunity.

J A Kans 1, M J Casadaban 1
PMCID: PMC209839  PMID: 2539356

Abstract

The Tn3 transposon inserts at a reduced frequency into a plasmid already containing a copy of Tn3, a phenomenon known as transposition immunity. The cis-acting site on Tn3 responsible for immunity was mapped by deletions from each side to be within the terminal 38-base-pair sequence that is inversely repeated at the ends of Tn3. Two palindromic sequences are present in the essential part of this region. Some deletions conferred only partial immunity, and others conferred negative immunity. Multiple copies of partially immune ends conferred additional immunity. No other part of Tn3 was necessary for immunity.

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Selected References

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  1. Adzuma K., Mizuuchi K. Target immunity of Mu transposition reflects a differential distribution of Mu B protein. Cell. 1988 Apr 22;53(2):257–266. doi: 10.1016/0092-8674(88)90387-x. [DOI] [PubMed] [Google Scholar]
  2. Arthur A., Nimmo E., Hettle S., Sherratt D. Transposition and transposition immunity of transposon Tn3 derivatives having different ends. EMBO J. 1984 Aug;3(8):1723–1729. doi: 10.1002/j.1460-2075.1984.tb02038.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arthur A., Sherratt D. Dissection of the transposition process: a transposon-encoded site-specific recombination system. Mol Gen Genet. 1979 Oct 1;175(3):267–274. doi: 10.1007/BF00397226. [DOI] [PubMed] [Google Scholar]
  4. Bennett P. M., Richmond M. H. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli. J Bacteriol. 1976 Apr;126(1):1–6. doi: 10.1128/jb.126.1.1-6.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop R., Sherratt D. Transposon Tn1 intra-molecular transposition. Mol Gen Genet. 1984;196(1):117–122. doi: 10.1007/BF00334102. [DOI] [PubMed] [Google Scholar]
  6. Casadaban M. J., Chou J., Cohen S. N. Overproduction of the Tn3 transposition protein and its role in DNA transposition. Cell. 1982 Feb;28(2):345–354. doi: 10.1016/0092-8674(82)90352-x. [DOI] [PubMed] [Google Scholar]
  7. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  8. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chiang S. J., Clowes R. C. Intramolecular transposition and inversion in plasmid R6K. J Bacteriol. 1980 May;142(2):668–682. doi: 10.1128/jb.142.2.668-682.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chiang S. J., Jordan E., Clowes R. C. Intermolecular and intramolecular transposition and transposition immunity in Tn3 and Tn2660. Mol Gen Genet. 1982;187(2):187–194. doi: 10.1007/BF00331116. [DOI] [PubMed] [Google Scholar]
  11. Chou J., Casadaban M. J., Lemaux P. G., Cohen S. N. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4020–4024. doi: 10.1073/pnas.76.8.4020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Chou J., Lemaux P. G., Casadaban M. J., Cohen S. N. Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product. Nature. 1979 Dec 20;282(5741):801–806. doi: 10.1038/282801a0. [DOI] [PubMed] [Google Scholar]
  13. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Craigie R., Mizuuchi K. Role of DNA topology in Mu transposition: mechanism of sensing the relative orientation of two DNA segments. Cell. 1986 Jun 20;45(6):793–800. doi: 10.1016/0092-8674(86)90554-4. [DOI] [PubMed] [Google Scholar]
  15. Datta N., Hedges R. W. Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. J Gen Microbiol. 1972 Sep;72(2):349–355. doi: 10.1099/00221287-72-2-349. [DOI] [PubMed] [Google Scholar]
  16. Faulds D., Dower N., Stahl M. M., Stahl F. W. Orientation-dependent recombination hotspot activity in bacteriophage lambda. J Mol Biol. 1979 Jul 15;131(4):681–695. doi: 10.1016/0022-2836(79)90197-9. [DOI] [PubMed] [Google Scholar]
  17. Fennewald M. A., Gerrard S. P., Chou J., Casadaban M. J., Cozzarelli N. R. Purification of the Tn3 transposase and analysis of its binding to DNA. J Biol Chem. 1981 May 25;256(10):4687–4690. [PubMed] [Google Scholar]
  18. Gill R. E., Heffron F., Falkow S. Identification of the protein encoded by the transposable element Tn3 which is required for its transposition. Nature. 1979 Dec 20;282(5741):797–801. doi: 10.1038/282797a0. [DOI] [PubMed] [Google Scholar]
  19. Gill R., Heffron F., Dougan G., Falkow S. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol. 1978 Nov;136(2):742–756. doi: 10.1128/jb.136.2.742-756.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Grindley N. D., Lauth M. R., Wells R. G., Wityk R. J., Salvo J. J., Reed R. R. Transposon-mediated site-specific recombination: identification of three binding sites for resolvase at the res sites of gamma delta and Tn3. Cell. 1982 Aug;30(1):19–27. doi: 10.1016/0092-8674(82)90007-1. [DOI] [PubMed] [Google Scholar]
  21. Grinsted J., Bennett P. M., Higginson S., Richmond M. H. Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Mol Gen Genet. 1978 Nov 9;166(3):313–320. doi: 10.1007/BF00267624. [DOI] [PubMed] [Google Scholar]
  22. Guyer M. S., Reed R. R., Steitz J. A., Low K. B. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):135–140. doi: 10.1101/sqb.1981.045.01.022. [DOI] [PubMed] [Google Scholar]
  23. Harshey R. M., Getzoff E. D., Baldwin D. L., Miller J. L., Chaconas G. Primary structure of phage mu transposase: homology to mu repressor. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7676–7680. doi: 10.1073/pnas.82.22.7676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Heffron F., Bedinger P., Champoux J. J., Falkow S. Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci U S A. 1977 Feb;74(2):702–706. doi: 10.1073/pnas.74.2.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Heffron F., McCarthy B. J., Ohtsubo H., Ohtsubo E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell. 1979 Dec;18(4):1153–1163. doi: 10.1016/0092-8674(79)90228-9. [DOI] [PubMed] [Google Scholar]
  26. Heffron F., So M., McCarthy B. J. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6012–6016. doi: 10.1073/pnas.75.12.6012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Heritage J., Bennett P. M. The role of TnA transposase in transposition immunity. Plasmid. 1984 Nov;12(3):218–221. doi: 10.1016/0147-619x(84)90049-0. [DOI] [PubMed] [Google Scholar]
  28. Huang C. J., Heffron F., Twu J. S., Schloemer R. H., Lee C. H. Analysis of Tn3 sequences required for transposition and immunity. Gene. 1986;41(1):23–31. doi: 10.1016/0378-1119(86)90263-5. [DOI] [PubMed] [Google Scholar]
  29. Ichikawa H., Ikeda K., Wishart W. L., Ohtsubo E. Specific binding of transposase to terminal inverted repeats of transposable element Tn3. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8220–8224. doi: 10.1073/pnas.84.23.8220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Kitts P. A., Lamond A., Sherratt D. J. Inter-replicon transposition of Tn1/3 occurs in two sequential genetically separable steps. Nature. 1982 Feb 18;295(5850):626–628. doi: 10.1038/295626a0. [DOI] [PubMed] [Google Scholar]
  31. Kitts P., Symington L., Burke M., Reed R., Sherratt D. Transposon-specified site-specific recombination. Proc Natl Acad Sci U S A. 1982 Jan;79(1):46–50. doi: 10.1073/pnas.79.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Kopecko D. J., Cohen S. N. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373–1377. doi: 10.1073/pnas.72.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Kostriken R., Morita C., Heffron F. Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4041–4045. doi: 10.1073/pnas.78.7.4041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Kretschmer P. J., Cohen S. N. Effect of temperature on translocation frequency of the Tn3 element. J Bacteriol. 1979 Aug;139(2):515–519. doi: 10.1128/jb.139.2.515-519.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Kretschmer P. J., Cohen S. N. Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J Bacteriol. 1977 May;130(2):888–899. doi: 10.1128/jb.130.2.888-899.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Kupersztoch Y. M., Helinski D. R. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451–1459. doi: 10.1016/0006-291x(73)91149-2. [DOI] [PubMed] [Google Scholar]
  37. Lee C. H., Bhagwat A., Heffron F. Identification of a transposon Tn3 sequence required for transposition immunity. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6765–6769. doi: 10.1073/pnas.80.22.6765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Marinus M. G. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet. 1973 Dec 14;127(1):47–55. doi: 10.1007/BF00267782. [DOI] [PubMed] [Google Scholar]
  39. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. McCormick M., Wishart W., Ohtsubo H., Heffron F., Ohtsubo E. Plasmid cointegrates and their resolution mediated by transposon Tn3 mutants. Gene. 1981 Nov;15(2-3):103–118. doi: 10.1016/0378-1119(81)90120-7. [DOI] [PubMed] [Google Scholar]
  41. Muster C. J., MacHattie L. A., Shapiro J. A. p lambda CM system: observations on the roles of transposable elements in formation and breakdown of plasmids derived from bacteriophage lambda replicons. J Bacteriol. 1983 Feb;153(2):976–990. doi: 10.1128/jb.153.2.976-990.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. New J. H., Eggleston A. K., Fennewald M. Binding of the Tn3 transposase to the inverted repeats of Tn3. J Mol Biol. 1988 Jun 5;201(3):589–599. doi: 10.1016/0022-2836(88)90640-7. [DOI] [PubMed] [Google Scholar]
  43. Nisen P. D., Kopecko D. J., Chou J., Cohen S. N. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J Mol Biol. 1977 Dec 25;117(4):975–978. doi: 10.1016/s0022-2836(77)80008-9. [DOI] [PubMed] [Google Scholar]
  44. Reed R. R. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428–3432. doi: 10.1073/pnas.78.6.3428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Reed R. R. Transposon-mediated site-specific recombination: a defined in vitro system. Cell. 1981 Sep;25(3):713–719. doi: 10.1016/0092-8674(81)90178-1. [DOI] [PubMed] [Google Scholar]
  46. Robinson M. K., Bennett P. M., Richmond M. H. Inhibition of TnA translocation by TnA. J Bacteriol. 1977 Jan;129(1):407–414. doi: 10.1128/jb.129.1.407-414.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Shapira S. K., Chou J., Richaud F. V., Casadaban M. J. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase. Gene. 1983 Nov;25(1):71–82. doi: 10.1016/0378-1119(83)90169-5. [DOI] [PubMed] [Google Scholar]
  48. Shapiro J. A. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933–1937. doi: 10.1073/pnas.76.4.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Simpson R. B. Interaction of the cAMP receptor protein with the lac promoter. Nucleic Acids Res. 1980 Feb 25;8(4):759–766. [PMC free article] [PubMed] [Google Scholar]
  50. Soberon X., Covarrubias L., Bolivar F. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980 May;9(3-4):287–305. doi: 10.1016/0378-1119(90)90328-o. [DOI] [PubMed] [Google Scholar]
  51. Spengler S. J., Stasiak A., Cozzarelli N. R. The stereostructure of knots and catenanes produced by phage lambda integrative recombination: implications for mechanism and DNA structure. Cell. 1985 Aug;42(1):325–334. doi: 10.1016/s0092-8674(85)80128-8. [DOI] [PubMed] [Google Scholar]
  52. Tu C. P., Cohen S. N. Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell. 1980 Jan;19(1):151–160. doi: 10.1016/0092-8674(80)90396-7. [DOI] [PubMed] [Google Scholar]
  53. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  54. Vosberg H. P., Hoffmann-Berling H. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J Mol Biol. 1971 Jun 28;58(3):739–753. doi: 10.1016/0022-2836(71)90037-4. [DOI] [PubMed] [Google Scholar]
  55. Wallace L. J., Ward J. M., Bennett P. M., Robinson M. K., Richmond M. H. Transposition immunity. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):183–188. doi: 10.1101/sqb.1981.045.01.029. [DOI] [PubMed] [Google Scholar]
  56. Wallace L. J., Ward J. M., Richmond M. H. The location of sequences of TnA required for the establishment of transposition immunity. Mol Gen Genet. 1981;184(1):80–86. doi: 10.1007/BF00271199. [DOI] [PubMed] [Google Scholar]
  57. Yuan R. Structure and mechanism of multifunctional restriction endonucleases. Annu Rev Biochem. 1981;50:285–319. doi: 10.1146/annurev.bi.50.070181.001441. [DOI] [PubMed] [Google Scholar]

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