Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Apr;171(4):2226–2228. doi: 10.1128/jb.171.4.2226-2228.1989

Major outer membrane proteins unique to reproductive cells of Hyphomonas jannaschiana.

N Shen 1, L Dagasan 1, D Sledjeski 1, R M Weiner 1
PMCID: PMC209883  PMID: 2703471

Abstract

Separation on the basis of molecular weight resolved three proteins specific to the swarmer cell of Hyphomonas jannaschiana. In the reproductive cell, 4 major proteins were identified as cytoplasmic and 10 were identified as envelope. Of these envelope proteins, one was common to both the inner and outer membranes, four were common to the inner membrane, and five were common to the outer membrane. Four of these outer membrane proteins were specific to the reproductive cell, and two of these proteins, with apparent molecular weights of 116,000 and 29,000, constituted 19% of the total cell protein and 54% of the outer membrane protein.

Full text

PDF
2226

Images in this article

2227Image
on p.2227

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F., Nikaido K. Two-dimensional gel electrophoresis of membrane proteins. Biochemistry. 1976 Feb 10;15(3):616–623. doi: 10.1021/bi00648a026. [DOI] [PubMed] [Google Scholar]
  2. Auran T. B., Schmidt E. L. Similarities between Hyphomicrobium and Nitrobacter with respect to fatty acids. J Bacteriol. 1972 Jan;109(1):450–451. doi: 10.1128/jb.109.1.450-451.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berenguer J., Faraldo M. L., de Pedro M. A. Ca2+-stabilized oligomeric protein complexes are major components of the cell envelope of "Thermus thermophilus" HB8. J Bacteriol. 1988 Jun;170(6):2441–2447. doi: 10.1128/jb.170.6.2441-2447.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. DiRienzo J. M., Nakamura K., Inouye M. The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions. Annu Rev Biochem. 1978;47:481–532. doi: 10.1146/annurev.bi.47.070178.002405. [DOI] [PubMed] [Google Scholar]
  6. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  7. Goldfine H., Hagen P. N-methyl groups in bacterial lipids. 3. Phospholipids of hyphomicrobia. J Bacteriol. 1968 Feb;95(2):367–375. doi: 10.1128/jb.95.2.367-375.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hagen P. O., Goldfine H., Williams P. J. Phospholipids of bacteria with extensive intracytoplasmic membranes. Science. 1966 Mar 25;151(3717):1543–1544. doi: 10.1126/science.151.3717.1543. [DOI] [PubMed] [Google Scholar]
  9. Jannasch H. W., Wirsen C. O. Morphological survey of microbial mats near deep-sea thermal vents. Appl Environ Microbiol. 1981 Feb;41(2):528–538. doi: 10.1128/aem.41.2.528-538.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  11. Moore R. L. The biology of Hyphomicrobium and other prosthecate, budding bacteria. Annu Rev Microbiol. 1981;35:567–594. doi: 10.1146/annurev.mi.35.100181.003031. [DOI] [PubMed] [Google Scholar]
  12. Park C. E., Berger L. R. Complex lipids of Rhodomicrobium vannielii. J Bacteriol. 1967 Jan;93(1):221–229. doi: 10.1128/jb.93.1.221-229.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Switzer R. C., 3rd, Merril C. R., Shifrin S. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem. 1979 Sep 15;98(1):231–237. doi: 10.1016/0003-2697(79)90732-2. [DOI] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wali T. M., Hudson G. R., Danald D. A., Weiner R. M. Timing of swarmer cell cycle morphogenesis and macromolecular synthesis by Hyphomicrobium neptunium in synchronous culture. J Bacteriol. 1980 Oct;144(1):406–412. doi: 10.1128/jb.144.1.406-412.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weiner R. M., Blackman M. A. Inhibition of deoxyribonucleic acid synthesis and bud formation by nalidixic acid in Hyphomicrobium neptunium. J Bacteriol. 1973 Dec;116(3):1398–1404. doi: 10.1128/jb.116.3.1398-1404.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES