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. 1989 Apr;171(4):2238–2240. doi: 10.1128/jb.171.4.2238-2240.1989

Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings.

A Marra 1, H A Shuman 1
PMCID: PMC209886  PMID: 2703472

Abstract

The ability of Legionella pneumophila to act as a recipient of IncP and IncQ plasmids in matings with Escherichia coli varies widely from strain to strain. We found that the low efficiency of mating of the Philadelphia-1 strain is due to a type II restriction-modification system, and we isolated and characterized a Philadelphia-1 mutant that lacks the restriction enzyme activity.

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Selected References

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