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. 1989 May;171(5):2474–2479. doi: 10.1128/jb.171.5.2474-2479.1989

Size and physical map of the chromosome of Haemophilus influenzae.

L Kauc 1, M Mitchell 1, S H Goodgal 1
PMCID: PMC209923  PMID: 2785105

Abstract

A variation of pulse-field electrophoresis, field-inversion gel electrophoresis, was used to determine the size and physical map of the chromosome of Haemophilus influenzae. The DNA of H. influenzae had a low G + C content (39%) and no restriction sites for the enzymes NotI or SfiI. However, a number of restriction enzymes (SmaI, ApaI, NaeI, and SacII) that recognized 6-base-pair sequences containing only G and C nucleotides were found to generate a reasonable number of DNA fragments that were separable in agarose gels by field-inversion gel electrophoresis. The sizes of the DNA fragments were calibrated with a lambda DNA ladder and lambda DNA restriction fragments. The sum of fragment sizes obtained with restriction digests yielded a value for the chromosome of 1,980 kilobase pairs. Hybridization of a labeled fragment with two or more fragments from a digest with a different restriction enzyme provided the information needed to construct a circular map of the H. influenzae chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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