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. 1989 May;171(5):2756–2761. doi: 10.1128/jb.171.5.2756-2761.1989

Mutational changes in physiochemical cell surface properties of plant-growth-stimulating Pseudomonas spp. do not influence the attachment properties of the cells.

L A de Weger 1, M C van Loosdrecht 1, H E Klaassen 1, B Lugtenberg 1
PMCID: PMC209961  PMID: 2708317

Abstract

Bacteriophage-resistant mutant strains of the root-colonizing Pseudomonas strains WCS358 and WCS374 lack the O-antigenic side chain of the lipopolysaccharide, as was shown by the loss of the typical lipopolysaccharide ladder pattern after analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These strains differed from their parent strains in cell surface hydrophobicity and in cell surface charge. The observed variation in these physicochemical characteristics could be explained by the differences in sugar composition. The mutant strains had no altered properties of adherence to sterile potato roots compared with their parental strains, nor were differences observed in the firm adhesion to hydrophilic, lipophilic, negatively charged, or positively charged artificial surfaces. These results show that neither physicochemical cell surface properties nor the presence of the O-antigenic side chain plays a major role in the firm adhesion of these bacterial cells to solid surfaces, including potato roots.

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Selected References

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  1. Anderson A. J., Habibzadegah-Tari P., Tepper C. S. Molecular Studies on the Role of a Root Surface Agglutinin in Adherence and Colonization by Pseudomonas putida. Appl Environ Microbiol. 1988 Feb;54(2):375–380. doi: 10.1128/aem.54.2.375-380.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Weger L. A., van der Vlugt C. I., Wijfjes A. H., Bakker P. A., Schippers B., Lugtenberg B. Flagella of a plant-growth-stimulating Pseudomonas fluorescens strain are required for colonization of potato roots. J Bacteriol. 1987 Jun;169(6):2769–2773. doi: 10.1128/jb.169.6.2769-2773.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. James D. W., Suslow T. V., Steinback K. E. Relationship between Rapid, Firm Adhesion and Long-Term Colonization of Roots by Bacteria. Appl Environ Microbiol. 1985 Aug;50(2):392–397. doi: 10.1128/aem.50.2.392-397.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  6. Labischinski H., Barnickel G., Bradaczek H., Naumann D., Rietschel E. T., Giesbrecht P. High state of order of isolated bacterial lipopolysaccharide and its possible contribution to the permeation barrier property of the outer membrane. J Bacteriol. 1985 Apr;162(1):9–20. doi: 10.1128/jb.162.1.9-20.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lam J. S., Lam M. Y., MacDonald L. A., Hancock R. E. Visualization of Pseudomonas aeruginosa O antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin M monoclonal antibodies. J Bacteriol. 1987 Aug;169(8):3531–3538. doi: 10.1128/jb.169.8.3531-3538.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lindow S. E. Competitive Exclusion of Epiphytic Bacteria by IcePseudomonas syringae Mutants. Appl Environ Microbiol. 1987 Oct;53(10):2520–2527. doi: 10.1128/aem.53.10.2520-2527.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  10. Lugtenberg B., Van Alphen L. Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria. Biochim Biophys Acta. 1983 Mar 21;737(1):51–115. doi: 10.1016/0304-4157(83)90014-x. [DOI] [PubMed] [Google Scholar]
  11. Rivera M., Bryan L. E., Hancock R. E., McGroarty E. J. Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length. J Bacteriol. 1988 Feb;170(2):512–521. doi: 10.1128/jb.170.2.512-521.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  13. de Weger L. A., Jann B., Jann K., Lugtenberg B. Lipopolysaccharides of Pseudomonas spp. that stimulate plant growth: composition and use for strain identification. J Bacteriol. 1987 Apr;169(4):1441–1446. doi: 10.1128/jb.169.4.1441-1446.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. de Weger L. A., van Boxtel R., van der Burg B., Gruters R. A., Geels F. P., Schippers B., Lugtenberg B. Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp. J Bacteriol. 1986 Feb;165(2):585–594. doi: 10.1128/jb.165.2.585-594.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Loosdrecht M. C., Lyklema J., Norde W., Schraa G., Zehnder A. J. Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion. Appl Environ Microbiol. 1987 Aug;53(8):1898–1901. doi: 10.1128/aem.53.8.1898-1901.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van Loosdrecht M. C., Lyklema J., Norde W., Schraa G., Zehnder A. J. The role of bacterial cell wall hydrophobicity in adhesion. Appl Environ Microbiol. 1987 Aug;53(8):1893–1897. doi: 10.1128/aem.53.8.1893-1897.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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