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. 1989 May;171(5):2873–2875. doi: 10.1128/jb.171.5.2873-2875.1989

Electron transport and electrochemical proton gradient in membrane vesicles of Clostridium thermoautotrophicum.

J Hugenholtz 1, L G Ljungdahl 1
PMCID: PMC209977  PMID: 2708323

Abstract

Membrane vesicles of Clostridium thermoautotrophicum containing carbon monoxide dehydrogenase generated a proton motive force when exposed to CO. This proton motive force, with a value of -140 mV, consisted of only an electrical potential at pH 7.5 and above and of an electrical potential and pH gradient at a lower pH. The proton motive force drove the uptake of L-alanine by the vesicles to a concentration of 300 times that of the medium.

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Selected References

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