Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 May;171(5):2876–2878. doi: 10.1128/jb.171.5.2876-2878.1989

Novel arrangement of rRNA genes in Mycoplasma gallisepticum: separation of the 16S gene of one set from the 23S and 5S genes.

X Chen 1, L R Finch 1
PMCID: PMC209978  PMID: 2708324

Abstract

Large restriction fragments from the DNA of Mycoplasma gallisepticum S6 and PG31, which were prepared by digestion with BglI, BssHII, SmaI, or XhoI and which were separated by pulsed-field electrophoresis, were hybridized with probes containing most, or different parts, of an rRNA operon of Mycoplasma capricolum. The results showed that the genomes contained three widely separated rRNA loci. One locus contained genes for all three rRNA species and another contained 23S and probably 5S rRNA genes, whereas the third appeared to have only a 16S rRNA gene.

Full text

PDF
2876

Images in this article

2877Image
on p.2877

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amikam D., Glaser G., Razin S. Mycoplasmas (Mollicutes) have a low number of rRNA genes. J Bacteriol. 1984 Apr;158(1):376–378. doi: 10.1128/jb.158.1.376-378.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amikam D., Razin S., Glaser G. Ribosomal RNA genes in Mycoplasma. Nucleic Acids Res. 1982 Jul 24;10(14):4215–4222. doi: 10.1093/nar/10.14.4215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carle G. F., Frank M., Olson M. V. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science. 1986 Apr 4;232(4746):65–68. doi: 10.1126/science.3952500. [DOI] [PubMed] [Google Scholar]
  4. Maniloff J. Evolution of wall-less prokaryotes. Annu Rev Microbiol. 1983;37:477–499. doi: 10.1146/annurev.mi.37.100183.002401. [DOI] [PubMed] [Google Scholar]
  5. Pyle L. E., Corcoran L. N., Cocks B. G., Bergemann A. D., Whitley J. C., Finch L. R. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes. Nucleic Acids Res. 1988 Jul 11;16(13):6015–6025. doi: 10.1093/nar/16.13.6015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pyle L. E., Finch L. R. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988 Jul 11;16(13):6027–6039. doi: 10.1093/nar/16.13.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pyle L. E., Finch L. R. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA. Nucleic Acids Res. 1988 Mar 25;16(5):2263–2268. doi: 10.1093/nar/16.5.2263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Razin S., Yogev D. Genetic relatedness among Ureaplasma urealyticum serotypes (serovars). Pediatr Infect Dis. 1986 Nov-Dec;5(6 Suppl):S300–S304. doi: 10.1097/00006454-198611010-00022. [DOI] [PubMed] [Google Scholar]
  10. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taschke C., Klinkert M. Q., Wolters J., Herrmann R. Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: the 5S rRNA gene is separated from the 16S and 23S rRNA genes. Mol Gen Genet. 1986 Dec;205(3):428–433. doi: 10.1007/BF00338078. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES