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. 1989 May;171(5):2903–2905. doi: 10.1128/jb.171.5.2903-2905.1989

Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein.

J Grodberg 1, J J Dunn 1
PMCID: PMC209985  PMID: 2651422

Abstract

The predicted amino acid sequence of OmpT, an Escherichia coli outer membrane protease, was found to be highly homologous to that predicted for the pgtE gene product of Salmonella typhimurium. In this paper, it is shown that pgtE codes for a protein functionally homologous to OmpT as judged by its ability to proteolyze T7 RNA polymerase and to localize in the outer membrane of E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benson S. A., Hall M. N., Silhavy T. J. Genetic analysis of protein export in Escherichia coli K12. Annu Rev Biochem. 1985;54:101–134. doi: 10.1146/annurev.bi.54.070185.000533. [DOI] [PubMed] [Google Scholar]
  2. Davanloo P., Rosenberg A. H., Dunn J. J., Studier F. W. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035–2039. doi: 10.1073/pnas.81.7.2035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Delbaere L. T., Hutcheon W. L., James M. N., Thiessen W. E. Tertiary structural differences between microbial serine proteases and pancreatic serine enzymes. Nature. 1975 Oct 30;257(5529):758–763. doi: 10.1038/257758a0. [DOI] [PubMed] [Google Scholar]
  4. Gayda R. C., Markovitz A. Cloned DNA fragment specifying major outer membrane protein a in Escherichia coli K-12. J Bacteriol. 1978 Oct;136(1):369–380. doi: 10.1128/jb.136.1.369-380.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goff S. A., Goldberg A. L. An increased content of protease La, the lon gene product, increases protein degradation and blocks growth in Escherichia coli. J Biol Chem. 1987 Apr 5;262(10):4508–4515. [PubMed] [Google Scholar]
  6. Gordon G., Gayda R. C., Markovitz A. Sequence of the regulatory region of omp T, the gene specifying major outer membrane protein a (3b) of Escherichia coli K-12: implications for regulation and processing. Mol Gen Genet. 1984;193(3):414–421. doi: 10.1007/BF00382077. [DOI] [PubMed] [Google Scholar]
  7. Grodberg J., Lundrigan M. D., Toledo D. L., Mangel W. F., Dunn J. J. Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12. Nucleic Acids Res. 1988 Feb 11;16(3):1209–1209. doi: 10.1093/nar/16.3.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hollifield W. C., Jr, Fiss E. H., Neilands J. B. Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coli. Biochem Biophys Res Commun. 1978 Jul 28;83(2):739–746. doi: 10.1016/0006-291x(78)91051-3. [DOI] [PubMed] [Google Scholar]
  9. Oliver D. B., Beckwith J. Regulation of a membrane component required for protein secretion in Escherichia coli. Cell. 1982 Aug;30(1):311–319. doi: 10.1016/0092-8674(82)90037-x. [DOI] [PubMed] [Google Scholar]
  10. Oliver D. Protein secretion in Escherichia coli. Annu Rev Microbiol. 1985;39:615–648. doi: 10.1146/annurev.mi.39.100185.003151. [DOI] [PubMed] [Google Scholar]
  11. Pacaud M. Identification and localization of two membrane-bound esterases from Escherichia coli. J Bacteriol. 1982 Jan;149(1):6–14. doi: 10.1128/jb.149.1.6-14.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pacaud M., Richaud C. Protease II from Escherichia coli. Purification and characterization. J Biol Chem. 1975 Oct 10;250(19):7771–7779. [PubMed] [Google Scholar]
  13. Sugimura K., Nishihara T. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. J Bacteriol. 1988 Dec;170(12):5625–5632. doi: 10.1128/jb.170.12.5625-5632.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Swamy K. H., Goldberg A. L. Subcellular distribution of various proteases in Escherichia coli. J Bacteriol. 1982 Mar;149(3):1027–1033. doi: 10.1128/jb.149.3.1027-1033.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tabor S., Richardson C. C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074–1078. doi: 10.1073/pnas.82.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolfe P. B., Zwizinski C., Wickner W. Purification and characterization of leader peptidase from Escherichia coli. Methods Enzymol. 1983;97:40–46. doi: 10.1016/0076-6879(83)97116-1. [DOI] [PubMed] [Google Scholar]
  17. Yu G. Q., Hong J. S. Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium. Gene. 1986;45(1):51–57. doi: 10.1016/0378-1119(86)90131-9. [DOI] [PubMed] [Google Scholar]

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