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. 1989 Jun;171(6):3053–3059. doi: 10.1128/jb.171.6.3053-3059.1989

Genetic reconstitution of the high-affinity L-arabinose transport system.

B F Horazdovsky 1, R W Hogg 1
PMCID: PMC210014  PMID: 2656640

Abstract

Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding sequences was necessary to restore high-affinity L-arabinose transport. Kinetic analysis of this genetically reconstituted transport system indicated that it functions with essentially wild-type parameters. Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Bacterial periplasmic transport systems: structure, mechanism, and evolution. Annu Rev Biochem. 1986;55:397–425. doi: 10.1146/annurev.bi.55.070186.002145. [DOI] [PubMed] [Google Scholar]
  2. Ames G. F. Structure and mechanism of bacterial periplasmic transport systems. J Bioenerg Biomembr. 1988 Feb;20(1):1–18. doi: 10.1007/BF00762135. [DOI] [PubMed] [Google Scholar]
  3. Brown C. E., Hogg R. W. A second transport system for L-arabinose in Escherichia coli B-r controlled by the araC gene. J Bacteriol. 1972 Aug;111(2):606–613. doi: 10.1128/jb.111.2.606-613.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark A. F., Hogg R. W. High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location. J Bacteriol. 1981 Sep;147(3):920–924. doi: 10.1128/jb.147.3.920-924.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engstrom J., Wong A., Maurer R. Interaction of DNA polymerase III gamma and beta subunits in vivo in Salmonella typhimurium. Genetics. 1986 Jul;113(3):499–515. doi: 10.1093/genetics/113.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  7. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  8. Higgins C. F., Haag P. D., Nikaido K., Ardeshir F., Garcia G., Ames G. F. Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium. Nature. 1982 Aug 19;298(5876):723–727. doi: 10.1038/298723a0. [DOI] [PubMed] [Google Scholar]
  9. Hofnung M. Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12. Genetics. 1974 Feb;76(2):169–184. doi: 10.1093/genetics/76.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hogg R. W. "In vivo" detection of L-arabinose-binding protein, CRM-negative mutants. J Bacteriol. 1971 Feb;105(2):604–608. doi: 10.1128/jb.105.2.604-608.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hogg R. W. L-Arabinose transport and the L-arabinose binding protein of Escherichia coli. J Supramol Struct. 1977;6(3):411–417. doi: 10.1002/jss.400060314. [DOI] [PubMed] [Google Scholar]
  12. Horazdovsky B. F., Hogg R. W. High-affinity L-arabinose transport operon. Gene product expression and mRNAs. J Mol Biol. 1987 Sep 5;197(1):27–35. doi: 10.1016/0022-2836(87)90606-1. [DOI] [PubMed] [Google Scholar]
  13. Kolodrubetz D., Schleif R. L-arabinose transport systems in Escherichia coli K-12. J Bacteriol. 1981 Nov;148(2):472–479. doi: 10.1128/jb.148.2.472-479.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kushner S. R., Nagaishi H., Templin A., Clark A. J. Genetic recombination in Escherichia coli: the role of exonuclease I. Proc Natl Acad Sci U S A. 1971 Apr;68(4):824–827. doi: 10.1073/pnas.68.4.824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MacPherson A. J., Jones-Mortimer M. C., Henderson P. J. Identification of the AraE transport protein of Escherichia coli. Biochem J. 1981 Apr 15;196(1):269–283. doi: 10.1042/bj1960269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maiden M. C., Davis E. O., Baldwin S. A., Moore D. C., Henderson P. J. Mammalian and bacterial sugar transport proteins are homologous. Nature. 1987 Feb 12;325(6105):641–643. doi: 10.1038/325641a0. [DOI] [PubMed] [Google Scholar]
  17. Novotny C. P., Englesberg E. The L-arabinose permease system in Escherichia coli B/r. Biochim Biophys Acta. 1966 Mar 28;117(1):217–230. doi: 10.1016/0304-4165(66)90169-3. [DOI] [PubMed] [Google Scholar]
  18. Raibaud O., Roa M., Braun-Breton C., Schwartz M. Structure of the malB region in Escherichia coli K12. I. Genetic map of the malK-lamB operon. Mol Gen Genet. 1979 Jul 24;174(3):241–248. doi: 10.1007/BF00267796. [DOI] [PubMed] [Google Scholar]
  19. Schleif R. An L-arabinose binding protein and arabinose permeation in Escherichia coli. J Mol Biol. 1969 Nov 28;46(1):185–196. doi: 10.1016/0022-2836(69)90065-5. [DOI] [PubMed] [Google Scholar]
  20. Scripture J. B., Voelker C., Miller S., O'Donnell R. T., Polgar L., Rade J., Horazdovsky B. F., Hogg R. W. High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products. J Mol Biol. 1987 Sep 5;197(1):37–46. doi: 10.1016/0022-2836(87)90607-3. [DOI] [PubMed] [Google Scholar]
  21. Silhavy T. J., Brickman E., Bassford P. J., Jr, Casadaban M. J., Shuman H. A., Schwartz V., Guarente L., Schwartz M., Beckwith J. R. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol Gen Genet. 1979 Jul 24;174(3):249–259. doi: 10.1007/BF00267797. [DOI] [PubMed] [Google Scholar]
  22. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]

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