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. 1989 Jun;171(6):3080–3084. doi: 10.1128/jb.171.6.3080-3084.1989

Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction.

P Matsushima 1, M A McHenney 1, R H Baltz 1
PMCID: PMC210018  PMID: 2542216

Abstract

We constructed nonrestricting strains of Streptomyces fradiae blocked in different steps in tylosin biosynthesis. Plasmid transformation frequencies were 10(3)- to 10(4)-fold higher and bacteriophage plating efficiencies were 10(4)- to 10(8)-fold higher in the nonrestricting strains than in the restricting strains. The efficiencies of transduction of plasmid pRHB101 in S. fradiae strains varied by over 1,000-fold, depending on growth conditions, and optimum transduction frequencies were obtained when cells were grown to mid-exponential phase at 39 degrees C. Under these conditions, restricting and nonrestricting strains were transduced at frequencies that differed by only two- to fivefold.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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