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. 1989 Jun;171(6):3108–3114. doi: 10.1128/jb.171.6.3108-3114.1989

Product of the Lactococcus lactis gene required for malolactic fermentation is homologous to a family of positive regulators.

P Renault 1, C Gaillardin 1, H Heslot 1
PMCID: PMC210022  PMID: 2498286

Abstract

Malolactic fermentation is a secondary fermentation that many lactic acid bacteria can carry out when L-malate is present in the medium. The activation of the malolactic system in Lactococcus lactis is mediated by a locus we call mleR. Induction of the genes necessary to perform malolactic fermentation occurs only in bacteria with a functional copy of mleR. The mleR gene consists of one open reading frame capable of coding for a protein with a calculated molecular mass of 33,813 daltons. The amino acid sequence of the predicted MleR gene product is homologous to that of positive activators in gram-negative bacteria: LysR, IlvY gene products of Escherichia coli, MetR, CysB of Salmonella typhimurium, AmpR of Enterobacter cloacae, NodD of Rhizobium sp., and TrpI of Pseudomonas aeruginosa.

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Selected References

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