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. 1989 Jun;171(6):3440–3448. doi: 10.1128/jb.171.6.3440-3448.1989

Localization and regulation of bacteriophage Mu promoters.

S F Stoddard 1, M M Howe 1
PMCID: PMC210069  PMID: 2524470

Abstract

Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early), PcM (repressor), and Pmom (late) promoters. A major signal upstream of C was first observed at 12 min and intensified thereafter with RNA from cts and C amber but not replication-defective prophages; these characteristics indicate that this signal arises from a middle promoter, which we designate Pm. With 20- and 40-min RNA, four additional major signals originated in the C-lys, F-G-I, N-P, and com-mom regions. These signals were missing with RNA from C amber and replication-defective prophages and therefore reflected the activity of late promoters, one of which we presume was Pmom. Uninduced lysogens showed weak signals from five regions, one from the early regulatory region, three between genes B and lys, and one near the late genes K, L, and M. The first of these probably resulted from PcM activity; the others remain to be identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazaral M., Helinski D. R. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185–194. doi: 10.1016/0022-2836(68)90374-4. [DOI] [PubMed] [Google Scholar]
  2. Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bukhari A. I., Ambrosio L. The invertible segment of bacteriophage Mu DNA determines the adsorption properties of Mu particles. Nature. 1978 Feb 9;271(5645):575–577. doi: 10.1038/271575a0. [DOI] [PubMed] [Google Scholar]
  4. Bölker M., Wulczyn F. G., Kahmann R. Role of bacteriophage Mu C protein in activation of the mom gene promoter. J Bacteriol. 1989 Apr;171(4):2019–2027. doi: 10.1128/jb.171.4.2019-2027.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giphart-Gassler M., Plasterk R. H., van de Putte P. G inversion in bacteriophage Mu: a novel way of gene splicing. Nature. 1982 May 27;297(5864):339–342. doi: 10.1038/297339a0. [DOI] [PubMed] [Google Scholar]
  6. Gloor G., Chaconas G. The bacteriophage Mu N gene encodes the 64-kDa virion protein which is injected with, and circularizes, infecting Mu DNA. J Biol Chem. 1986 Dec 15;261(35):16682–16688. [PubMed] [Google Scholar]
  7. Goosen N., van Heuvel M., Moolenaar G. F., van de Putte P. Regulation of Mu transposition. II. The escherichia coli HimD protein positively controls two repressor promoters and the early promoter of bacteriophage Mu. Gene. 1984 Dec;32(3):419–426. doi: 10.1016/0378-1119(84)90017-9. [DOI] [PubMed] [Google Scholar]
  8. Goosen T., Giphart-Gassler M., Van de Putte P. Bacteriophage Mu DNA replication is stimulated by non-essential early functions. Mol Gen Genet. 1982;186(1):135–139. doi: 10.1007/BF00422925. [DOI] [PubMed] [Google Scholar]
  9. Grundy F. J., Howe M. M. Involvement of the invertible G segment in bacteriophage mu tail fiber biosynthesis. Virology. 1984 Apr 30;134(2):296–317. doi: 10.1016/0042-6822(84)90299-x. [DOI] [PubMed] [Google Scholar]
  10. Grundy F. J., Howe M. M. Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu. Virology. 1985 Jun;143(2):485–504. doi: 10.1016/0042-6822(85)90388-5. [DOI] [PubMed] [Google Scholar]
  11. Hattman S., Ives J., Margolin W., Howe M. M. Regulation and expression of the bacteriophage mu mom gene: mapping of the transactivation (dad) function to the C region. Gene. 1985;39(1):71–76. doi: 10.1016/0378-1119(85)90109-x. [DOI] [PubMed] [Google Scholar]
  12. Heisig P., Kahmann R. The sequence and mom-transactivation function of the C gene of bacteriophage Mu. Gene. 1986;43(1-2):59–67. doi: 10.1016/0378-1119(86)90008-9. [DOI] [PubMed] [Google Scholar]
  13. Howe M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. doi: 10.1016/0042-6822(73)90118-9. [DOI] [PubMed] [Google Scholar]
  14. Howe M. M., Schumm J. W., Taylor A. L. The S and U genes of bacteriophage mu are located in the invertible G segment of mu DNA. Virology. 1979 Jan 15;92(1):108–124. doi: 10.1016/0042-6822(79)90218-6. [DOI] [PubMed] [Google Scholar]
  15. Kassavetis G. A., Geiduschek E. P. Bacteriophage T4 late promoters: mapping 5' ends of T4 gene 23 mRNAs. EMBO J. 1982;1(1):107–114. doi: 10.1002/j.1460-2075.1982.tb01132.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krause H. M., Higgins N. P. Positive and negative regulation of the Mu operator by Mu repressor and Escherichia coli integration host factor. J Biol Chem. 1986 Mar 15;261(8):3744–3752. [PubMed] [Google Scholar]
  17. Krause H. M., Rothwell M. R., Higgins N. P. The early promoter of bacteriophage Mu: definition of the site of transcript initiation. Nucleic Acids Res. 1983 Aug 25;11(16):5483–5495. doi: 10.1093/nar/11.16.5483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Margolin W., Howe M. M. Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription. Nucleic Acids Res. 1986 Jun 25;14(12):4881–4897. doi: 10.1093/nar/14.12.4881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Margolin W., Rao G., Howe M. M. Bacteriophage Mu late promoters: four late transcripts initiate near a conserved sequence. J Bacteriol. 1989 Apr;171(4):2003–2018. doi: 10.1128/jb.171.4.2003-2018.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Marrs C. F., Howe M. M. AvaII and BglI restriction maps of bacteriophage Mu. Virology. 1983 Apr 30;126(2):563–575. doi: 10.1016/s0042-6822(83)80013-0. [DOI] [PubMed] [Google Scholar]
  21. McKenney K., Shimatake H., Court D., Schmeissner U., Brady C., Rosenberg M. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif Anal. 1981;2:383–415. [PubMed] [Google Scholar]
  22. Pato M. L., Reich C. Instability of transposase activity: evidence from bacteriophage mu DNA replication. Cell. 1982 May;29(1):219–225. doi: 10.1016/0092-8674(82)90106-4. [DOI] [PubMed] [Google Scholar]
  23. Pato M. L., Reich C. Stoichiometric use of the transposase of bacteriophage Mu. Cell. 1984 Jan;36(1):197–202. doi: 10.1016/0092-8674(84)90089-8. [DOI] [PubMed] [Google Scholar]
  24. Salser W., Gesteland R. F., Bolle A. In vitro synthesis of bacteriophage lysozyme. Nature. 1967 Aug 5;215(5101):588–591. doi: 10.1038/215588a0. [DOI] [PubMed] [Google Scholar]
  25. Schumann W., Bade E. G., Forgie R. A., Howe M. M. Cloning of DNA fragments of the right end of phage mu and location of the HindIII, SalI, PstI, and BamHI restriction sites on the genetic map of mu. Virology. 1980 Jul 30;104(2):418–425. doi: 10.1016/0042-6822(80)90344-x. [DOI] [PubMed] [Google Scholar]
  26. Schumann W., Bade E. G. In vitro constructed plasmids containing both ends of bacteriophage Mu DNA express phage functions. Mol Gen Genet. 1979 Jan 16;169(1):97–105. doi: 10.1007/BF00267550. [DOI] [PubMed] [Google Scholar]
  27. Schumann W. Cloning and biological characterization of the immunity region of Escherichia coli phage Mu. Gene. 1979 Apr;5(4):275–290. doi: 10.1016/0378-1119(79)90103-3. [DOI] [PubMed] [Google Scholar]
  28. Schumm J. W., Moore D. D., Blattner F. R., Howe M. M. Correlation of the genetic and physical maps in the central region of the bacteriophage Mu genome. Virology. 1980 Aug;105(1):185–195. doi: 10.1016/0042-6822(80)90166-x. [DOI] [PubMed] [Google Scholar]
  29. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  30. Stoddard S. F., Howe M. M. DNA sequence within the Mu C operon. Nucleic Acids Res. 1987 Sep 11;15(17):7198–7198. doi: 10.1093/nar/15.17.7198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  32. Toussaint A., Lecocq J. P. Sensitivity of bacteriophage Mu-1 development to rifampicin and streptolydigin. Mol Gen Genet. 1974 Mar 14;129(2):185–188. doi: 10.1007/BF00268631. [DOI] [PubMed] [Google Scholar]
  33. Waggoner B. T., Pato M. L. Early events in the replication of Mu prophage DNA. J Virol. 1978 Sep;27(3):587–594. doi: 10.1128/jvi.27.3.587-594.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wijffelman C., Gassler M., Stevens W. F., van de Putte P. On the control of transcription of bacteriophage Mu. Mol Gen Genet. 1974;131(2):85–96. doi: 10.1007/BF00266145. [DOI] [PubMed] [Google Scholar]
  35. Wijffelman C., Lotterman B. Kinetics of Mu DNA synthesis. Mol Gen Genet. 1977 Mar 7;151(2):169–174. doi: 10.1007/BF00338691. [DOI] [PubMed] [Google Scholar]
  36. Wijffelman C., van de Putte P. Transcription of bacteriophage mu. An analysis of the transcription pattern in the early phase of phage development. Mol Gen Genet. 1974;135(4):327–337. doi: 10.1007/BF00271147. [DOI] [PubMed] [Google Scholar]

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