Abstract
The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize, or to carry out transposition.
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Selected References
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- Aitken A., Rouviere-Yaniv J. Amino and carboxy terminal sequences of the DNA-binding protein HU from the Cyanobacterium Synechocystis PCC 6701 (ATCC 27170). Biochem Biophys Res Commun. 1979 Nov 28;91(2):461–467. doi: 10.1016/0006-291x(79)91544-4. [DOI] [PubMed] [Google Scholar]
- Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broyles S. S., Pettijohn D. E. Interaction of the Escherichia coli HU protein with DNA. Evidence for formation of nucleosome-like structures with altered DNA helical pitch. J Mol Biol. 1986 Jan 5;187(1):47–60. doi: 10.1016/0022-2836(86)90405-5. [DOI] [PubMed] [Google Scholar]
- Craigie R., Arndt-Jovin D. J., Mizuuchi K. A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: protein and DNA substrate requirements. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7570–7574. doi: 10.1073/pnas.82.22.7570. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craigie R., Mizuuchi K. Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu. Cell. 1987 Nov 6;51(3):493–501. doi: 10.1016/0092-8674(87)90645-3. [DOI] [PubMed] [Google Scholar]
- Dixon N. E., Kornberg A. Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jan;81(2):424–428. doi: 10.1073/pnas.81.2.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drlica K., Rouviere-Yaniv J. Histonelike proteins of bacteria. Microbiol Rev. 1987 Sep;51(3):301–319. doi: 10.1128/mr.51.3.301-319.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dürrenberger M., Bjornsti M. A., Uetz T., Hobot J. A., Kellenberger E. Intracellular location of the histonelike protein HU in Escherichia coli. J Bacteriol. 1988 Oct;170(10):4757–4768. doi: 10.1128/jb.170.10.4757-4768.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faelen M., Toussaint A. Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage. Virology. 1973 Jul;54(1):117–124. doi: 10.1016/0042-6822(73)90121-9. [DOI] [PubMed] [Google Scholar]
- Funnell B. E., Baker T. A., Kornberg A. Complete enzymatic replication of plasmids containing the origin of the Escherichia coli chromosome. J Biol Chem. 1986 Apr 25;261(12):5616–5624. [PubMed] [Google Scholar]
- Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hedges R. W., Matthew M. Acquisition by Escherichia coli of plasmid-borne beta-lactamases normally confined to Pseudomonas spp. Plasmid. 1979 Apr;2(2):269–278. doi: 10.1016/0147-619x(79)90045-3. [DOI] [PubMed] [Google Scholar]
- Hiraga S., Niki H., Ogura T., Ichinose C., Mori H., Ezaki B., Jaffé A. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells. J Bacteriol. 1989 Mar;171(3):1496–1505. doi: 10.1128/jb.171.3.1496-1505.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaffé A., D'Ari R., Hiraga S. Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods. J Bacteriol. 1988 Jul;170(7):3094–3101. doi: 10.1128/jb.170.7.3094-3101.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jasin M., Schimmel P. Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments. J Bacteriol. 1984 Aug;159(2):783–786. doi: 10.1128/jb.159.2.783-786.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson R. C., Bruist M. F., Simon M. I. Host protein requirements for in vitro site-specific DNA inversion. Cell. 1986 Aug 15;46(4):531–539. doi: 10.1016/0092-8674(86)90878-0. [DOI] [PubMed] [Google Scholar]
- Kano Y., Osato K., Wada M., Imamoto F. Cloning and sequencing of the HU-2 gene of Escherichia coli. Mol Gen Genet. 1987 Sep;209(2):408–410. doi: 10.1007/BF00329674. [DOI] [PubMed] [Google Scholar]
- Kano Y., Wada M., Nagase T., Imamoto F. Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli. Gene. 1986;45(1):37–44. doi: 10.1016/0378-1119(86)90129-0. [DOI] [PubMed] [Google Scholar]
- Kano Y., Yoshino S., Wada M., Yokoyama K., Nobuhara M., Imamoto F. Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli. Mol Gen Genet. 1985;201(2):360–362. doi: 10.1007/BF00425687. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leach D., Symonds N. The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistance. Mol Gen Genet. 1979 May 4;172(2):179–184. doi: 10.1007/BF00268280. [DOI] [PubMed] [Google Scholar]
- Liebart J. C., Ghelardini P., Paolozzi L. Conservative integration of bacteriophage Mu DNA into pBR322 plasmid. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4362–4366. doi: 10.1073/pnas.79.14.4362. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morisato D., Kleckner N. Tn10 transposition and circle formation in vitro. Cell. 1987 Oct 9;51(1):101–111. doi: 10.1016/0092-8674(87)90014-6. [DOI] [PubMed] [Google Scholar]
- Murray K., Murray N. E. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. doi: 10.1016/s0022-2836(75)80086-6. [DOI] [PubMed] [Google Scholar]
- Norris V., Alliotte T., Jaffé A., D'Ari R. DNA replication termination in Escherichia coli parB (a dnaG allele), parA, and gyrB mutants affected in DNA distribution. J Bacteriol. 1986 Nov;168(2):494–504. doi: 10.1128/jb.168.2.494-504.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orr E., Fairweather N. F., Holland I. B., Pritchard R. H. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12. Mol Gen Genet. 1979;177(1):103–112. doi: 10.1007/BF00267259. [DOI] [PubMed] [Google Scholar]
- Roberts D., Hoopes B. C., McClure W. R., Kleckner N. IS10 transposition is regulated by DNA adenine methylation. Cell. 1985 Nov;43(1):117–130. doi: 10.1016/0092-8674(85)90017-0. [DOI] [PubMed] [Google Scholar]
- Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
- Ross W., Shore S. H., Howe M. M. Mutants of Escherichia coli defective for replicative transposition of bacteriophage Mu. J Bacteriol. 1986 Sep;167(3):905–919. doi: 10.1128/jb.167.3.905-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rouvière-Yaniv J., Gros F. Characterization of a novel, low-molecular-weight DNA-binding protein from Escherichia coli. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3428–3432. doi: 10.1073/pnas.72.9.3428. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rouvière-Yaniv J., Kjeldgaard N. O. Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett. 1979 Oct 15;106(2):297–300. doi: 10.1016/0014-5793(79)80518-9. [DOI] [PubMed] [Google Scholar]
- Rouvière-Yaniv J. Localization of the HU protein on the Escherichia coli nucleoid. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):439–447. doi: 10.1101/sqb.1978.042.01.047. [DOI] [PubMed] [Google Scholar]
- Rouvière-Yaniv J., Yaniv M., Germond J. E. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA. Cell. 1979 Jun;17(2):265–274. doi: 10.1016/0092-8674(79)90152-1. [DOI] [PubMed] [Google Scholar]
- Résibois A., Toussaint A., van Gijsegem F., Faelen M. Physical characterization of mini-mu and mini-D108. Gene. 1981 Jun-Jul;14(1-2):103–113. doi: 10.1016/0378-1119(81)90152-9. [DOI] [PubMed] [Google Scholar]
- Sanzey B. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase. J Bacteriol. 1979 Apr;138(1):40–47. doi: 10.1128/jb.138.1.40-47.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Storts D. R., Markovitz A. Construction and characterization of mutations in hupB, the gene encoding HU-beta (HU-1) in Escherichia coli K-12. J Bacteriol. 1988 Apr;170(4):1541–1547. doi: 10.1128/jb.170.4.1541-1547.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Surette M. G., Buch S. J., Chaconas G. Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA. Cell. 1987 Apr 24;49(2):253–262. doi: 10.1016/0092-8674(87)90566-6. [DOI] [PubMed] [Google Scholar]
- Toussaint A., Desmet L., Faelen M., Alazard R., Chandler M., Pato M. In vivo mutagenesis of bacteriophage Mu transposase. J Bacteriol. 1987 Dec;169(12):5700–5707. doi: 10.1128/jb.169.12.5700-5707.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toussaint A., Faelen M., Résibois A. Chromosomal rearrangements induced by mini-Mu and mini-D108: mini review and new data. Gene. 1981 Jun-Jul;14(1-2):115–119. doi: 10.1016/0378-1119(81)90153-0. [DOI] [PubMed] [Google Scholar]
- Varshavsky A. J., Nedospasov S. A., Bakayev V. V., Bakayeva T. G., Georgiev G. P. Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Res. 1977 Aug;4(8):2725–2745. doi: 10.1093/nar/4.8.2725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
- Wada M., Kano Y., Ogawa T., Okazaki T., Imamoto F. Construction and characterization of the deletion mutant of hupA and hupB genes in Escherichia coli. J Mol Biol. 1988 Dec 5;204(3):581–591. doi: 10.1016/0022-2836(88)90357-9. [DOI] [PubMed] [Google Scholar]