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. 1989 Jul;171(7):3996–4001. doi: 10.1128/jb.171.7.3996-4001.1989

Effects of variation of inverted-repeat sequences on reactions mediated by the transposase of Tn21.

C Martin 1, J Grinsted 1, F de la Cruz 1
PMCID: PMC210153  PMID: 2544566

Abstract

The frequencies of one-ended transposition and normal transposition of derivatives of Tn21 that contain mutant inverted-repeat sequences (IRs) have been measured. In general, there was a linear relationship between the log of the frequency of one-ended transposition of a mutant IR and the log of the frequency of normal transposition of an element flanked by a wild-type IR at one end and by the mutant IR at the other. This implied that one-ended and normal transposition share the rate-limiting step that determines the frequency of transposition and that both IRs are involved in the rate-limiting step in normal transposition. Surprisingly, it was found that only the outer 18 base pairs of the IR of Tn21 engaged accurately in both one-ended and normal transposition, at about 1% of the frequency of the wild-type IR.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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