Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Aug;171(8):4494–4497. doi: 10.1128/jb.171.8.4494-4497.1989

Saturation of mismatch repair in the mutD5 mutator strain of Escherichia coli.

V Damagnez 1, M P Doutriaux 1, M Radman 1
PMCID: PMC210230  PMID: 2666405

Abstract

The mutD (dnaQ) gene of Escherichia coli codes for the proofreading activity of DNA polymerase III. The very strong mutator phenotype of mutD5 strains seems to indicate that their postreplicational mismatch repair activity is also impaired. We show that the mismatch repair system of mutD5 strains is functional but saturated, presumably by the excess of DNA replication errors, since it is recovered by inhibiting chromosomal DNA replication. This recovery depends on de novo protein synthesis.

Full text

PDF
4494

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claverys J. P., Lacks S. A. Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria. Microbiol Rev. 1986 Jun;50(2):133–165. doi: 10.1128/mr.50.2.133-165.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox E. C. Bacterial mutator genes and the control of spontaneous mutation. Annu Rev Genet. 1976;10:135–156. doi: 10.1146/annurev.ge.10.120176.001031. [DOI] [PubMed] [Google Scholar]
  5. Degnen G. E., Cox E. C. Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies. J Bacteriol. 1974 Feb;117(2):477–487. doi: 10.1128/jb.117.2.477-487.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiFrancesco R., Bhatnagar S. K., Brown A., Bessman M. J. The interaction of DNA polymerase III and the product of the Escherichia coli mutator gene, mutD. J Biol Chem. 1984 May 10;259(9):5567–5573. [PubMed] [Google Scholar]
  7. Dohet C., Wagner R., Radman M. Repair of defined single base-pair mismatches in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Jan;82(2):503–505. doi: 10.1073/pnas.82.2.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Echols H., Lu C., Burgers P. M. Mutator strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoenzyme. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2189–2192. doi: 10.1073/pnas.80.8.2189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fersht A. R., Knill-Jones J. W., Tsui W. C. Kinetic basis of spontaneous mutation. Misinsertion frequencies, proofreading specificities and cost of proofreading by DNA polymerases of Escherichia coli. J Mol Biol. 1982 Mar 25;156(1):37–51. doi: 10.1016/0022-2836(82)90457-0. [DOI] [PubMed] [Google Scholar]
  10. Fowler R. G., Degnen G. E., Cox E. C. Mutational specificity of a conditional Escherichia coli mutator, mutD5. Mol Gen Genet. 1974;133(3):179–191. doi: 10.1007/BF00267667. [DOI] [PubMed] [Google Scholar]
  11. Fowler R. G., Schaaper R. M., Glickman B. W. Characterization of mutational specificity within the lacI gene for a mutD5 mutator strain of Escherichia coli defective in 3'----5' exonuclease (proofreading) activity. J Bacteriol. 1986 Jul;167(1):130–137. doi: 10.1128/jb.167.1.130-137.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gabay J., Yasunaka K. Interaction of the lamB protein with the peptidoglycan layer in Escherichia coli K12. Eur J Biochem. 1980 Feb;104(1):13–18. doi: 10.1111/j.1432-1033.1980.tb04393.x. [DOI] [PubMed] [Google Scholar]
  13. Guild W. R., Shoemaker N. B. Intracellular competition for a mismatch recogition system and marker-specific rescue of transforming DNA from inactivation by ultraviolet irradiation. Mol Gen Genet. 1974;128(4):291–300. doi: 10.1007/BF00268517. [DOI] [PubMed] [Google Scholar]
  14. Herman G. E., Modrich P. Escherichia coli K-12 clones that overproduce dam methylase are hypermutable. J Bacteriol. 1981 Jan;145(1):644–646. doi: 10.1128/jb.145.1.644-646.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jones M., Wagner R., Radman M. Repair of a mismatch is influenced by the base composition of the surrounding nucleotide sequence. Genetics. 1987 Apr;115(4):605–610. doi: 10.1093/genetics/115.4.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kunkel T. A. Exonucleolytic proofreading. Cell. 1988 Jun 17;53(6):837–840. doi: 10.1016/s0092-8674(88)90189-4. [DOI] [PubMed] [Google Scholar]
  17. Lindahl T., Demple B., Robins P. Suicide inactivation of the E. coli O6-methylguanine-DNA methyltransferase. EMBO J. 1982;1(11):1359–1363. doi: 10.1002/j.1460-2075.1982.tb01323.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marinus M. G., Morris N. R. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res. 1975 Apr;28(1):15–26. doi: 10.1016/0027-5107(75)90309-7. [DOI] [PubMed] [Google Scholar]
  19. Meselson M., Yuan R. DNA restriction enzyme from E. coli. Nature. 1968 Mar 23;217(5134):1110–1114. doi: 10.1038/2171110a0. [DOI] [PubMed] [Google Scholar]
  20. Modrich P. DNA mismatch correction. Annu Rev Biochem. 1987;56:435–466. doi: 10.1146/annurev.bi.56.070187.002251. [DOI] [PubMed] [Google Scholar]
  21. Monk M., Gross J. Induction of prophage lambda in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature. Mol Gen Genet. 1971;110(4):299–306. doi: 10.1007/BF00438272. [DOI] [PubMed] [Google Scholar]
  22. Radman M., Wagner R. Mismatch repair in Escherichia coli. Annu Rev Genet. 1986;20:523–538. doi: 10.1146/annurev.ge.20.120186.002515. [DOI] [PubMed] [Google Scholar]
  23. Schaaper R. M. Escherichia coli mutator mutD5 is defective in the mutHLS pathway of DNA mismatch repair. Genetics. 1989 Feb;121(2):205–212. doi: 10.1093/genetics/121.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schaaper R. M. Mechanisms of mutagenesis in the Escherichia coli mutator mutD5: role of DNA mismatch repair. Proc Natl Acad Sci U S A. 1988 Nov;85(21):8126–8130. doi: 10.1073/pnas.85.21.8126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schwartz M., Le Minor L. Occurrence of the bacteriophage lambda receptor in some enterobacteriaceae. J Virol. 1975 Apr;15(4):679–685. doi: 10.1128/jvi.15.4.679-685.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sevastopoulos C. G., Glaser D. A. Mutator action by Escherichia coli strains carrying dnaE mutations. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3947–3950. doi: 10.1073/pnas.74.9.3947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tiraby G., Fox M. S., Bernheimer H. Marker discrimination in deoxyribonucleic acid-mediated transformation of various Pneumococcus strains. J Bacteriol. 1975 Feb;121(2):608–618. doi: 10.1128/jb.121.2.608-618.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES