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. 1989 Aug;171(8):4521–4524. doi: 10.1128/jb.171.8.4521-4524.1989

Sporulation of Myxococcus xanthus in liquid shake flask cultures.

A Rosenbluh 1, E Rosenberg 1
PMCID: PMC210238  PMID: 2502539

Abstract

When suspended in a liquid starvation medium, exponentially growing Myxococcus xanthus sporulated within 3 days. These myxospores were similar to spores developed within fruiting bodies, as determined by electron microscopy and the production of spore-specific protein S. This liquid sporulation system may be useful as a means of preparing large quantities of myxospores and extracellular fluid for biochemical studies, including isolation of chemical signals produced during the sporulation process.

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Selected References

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  1. Bacon K., Eiserling F. A. A unique structure in microcysts of Myxococcus xanthus. J Ultrastruct Res. 1967 Dec;21(5):378–382. doi: 10.1016/s0022-5320(67)80147-3. [DOI] [PubMed] [Google Scholar]
  2. Bacon K., Rosenberg E. Ribonucleic acid synthesis during morphogenesis in Myxococcus xanthus. J Bacteriol. 1967 Dec;94(6):1883–1889. doi: 10.1128/jb.94.6.1883-1889.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burchard R. P. Myxospore induction in a nondispersed growing mutant of Myxococcus xanthus. J Bacteriol. 1975 Apr;122(1):302–306. doi: 10.1128/jb.122.1.302-306.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DWORKIN M., GIBSON S. M. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. doi: 10.1126/science.146.3641.243. [DOI] [PubMed] [Google Scholar]
  5. Downard J. S., Kupfer D., Zusman D. R. Gene expression during development of Myxococcus xanthus. Analysis of the genes for protein S. J Mol Biol. 1984 Jun 5;175(4):469–492. doi: 10.1016/0022-2836(84)90180-3. [DOI] [PubMed] [Google Scholar]
  6. Downard J. S., Zusman D. R. Differential expression of protein S genes during Myxococcus xanthus development. J Bacteriol. 1985 Mar;161(3):1146–1155. doi: 10.1128/jb.161.3.1146-1155.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dworkin M., Kaiser D. Cell interactions in myxobacterial growth and development. Science. 1985 Oct 4;230(4721):18–24. doi: 10.1126/science.3929384. [DOI] [PubMed] [Google Scholar]
  8. Gerisch G., Hess B. Cyclic-AMP-controlled oscillations in suspended Dictyostelium cells: their relation to morphogenetic cell interactions. Proc Natl Acad Sci U S A. 1974 May;71(5):2118–2122. doi: 10.1073/pnas.71.5.2118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hagen D. C., Bretscher A. P., Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978 Jun;64(2):284–296. doi: 10.1016/0012-1606(78)90079-9. [DOI] [PubMed] [Google Scholar]
  10. Inouye M., Inouye S., Zusman D. R. Biosynthesis and self-assembly of protein S, a development-specific protein of Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):209–213. doi: 10.1073/pnas.76.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inouye M., Inouye S., Zusman D. R. Gene expression during development of Myxococcus xanthus: pattern of protein synthesis. Dev Biol. 1979 Feb;68(2):579–591. doi: 10.1016/0012-1606(79)90228-8. [DOI] [PubMed] [Google Scholar]
  12. Janssen G. R., Dworkin M. Cell-cell interactions in developmental lysis of Myxococcus xanthus. Dev Biol. 1985 Nov;112(1):194–202. doi: 10.1016/0012-1606(85)90133-2. [DOI] [PubMed] [Google Scholar]
  13. Kaiser D. Social gliding is correlated with the presence of pili in Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5952–5956. doi: 10.1073/pnas.76.11.5952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuner J. M., Kaiser D. Fruiting body morphogenesis in submerged cultures of Myxococcus xanthus. J Bacteriol. 1982 Jul;151(1):458–461. doi: 10.1128/jb.151.1.458-461.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuspa A., Kroos L., Kaiser D. Intercellular signaling is required for developmental gene expression in Myxococcus xanthus. Dev Biol. 1986 Sep;117(1):267–276. doi: 10.1016/0012-1606(86)90369-6. [DOI] [PubMed] [Google Scholar]
  16. Qualls G. T., Stephens K., White D. Light-stimulated morphogenesis in the fruiting myxobacterium Stigmatella aurantiaca. Science. 1978 Aug 4;201(4354):444–445. doi: 10.1126/science.96528. [DOI] [PubMed] [Google Scholar]
  17. Sadler W., Dworkin M. Induction of cellular morphogenesis in Myxococcus xanthus. II. Macromolecular synthesis and mechanism of inducer action. J Bacteriol. 1966 Apr;91(4):1520–1525. doi: 10.1128/jb.91.4.1520-1525.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shimkets L. J. Role of cell cohesion in Myxococcus xanthus fruiting body formation. J Bacteriol. 1986 Jun;166(3):842–848. doi: 10.1128/jb.166.3.842-848.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stephens K., Hegeman G. D., White D. Pheromone produced by the myxobacterium Stigmatella aurantiaca. J Bacteriol. 1982 Feb;149(2):739–747. doi: 10.1128/jb.149.2.739-747.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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