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. 1989 Sep;171(9):4674–4678. doi: 10.1128/jb.171.9.4674-4678.1989

Identification and characterization of a cyanate permease in Escherichia coli K-12.

Y C Sung 1, J A Fuchs 1
PMCID: PMC210266  PMID: 2670891

Abstract

Escherichia coli contains an inducible enzyme, cyanase, that catalyzes the decomposition of cyanate into ammonia and bicarbonate. The gene encoding cyanase, cynS, was cloned and found to be on a DNA fragment that contained the lac operon. Characterization of a plasmid encoding cyanase indicated that a 26-kilodalton (kDa) protein of unknown function was also induced by cyanate (Y-C. Sung, D. Parsell, P.M. Anderson, and J.A. Fuchs, J. Bacteriol. 169:2639-2642, 1987). The gene encoding the 26-kDa protein was located between cynS and its promoter, indicating the existence of a cyn operon. The 26-kDa protein was identified as a cyanate permease that transports exogenous cyanate by active transport. E. coli was shown to contain a cyanate transport system that is energy dependent and saturable by cyanate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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