Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Sep;171(9):4742–4751. doi: 10.1128/jb.171.9.4742-4751.1989

Magnesium transport in Salmonella typhimurium: genetic characterization and cloning of three magnesium transport loci.

S P Hmiel 1, M D Snavely 1, J B Florer 1, M E Maguire 1, C G Miller 1
PMCID: PMC210275  PMID: 2548998

Abstract

Salmonella typhimurium strains lacking the CorA Mg2+ transport system retain Mg2+ transport and the ability to grow in medium containing a low concentration of Mg2+. Mutagenesis of a corA strain followed by ampicillin selection allowed isolation of a strain that required Mg2+-supplemented media for growth. This strain contained mutations in at least two loci in addition to corA, designated mgtA and mgtB (for magnesium transport). Strains with mutations at all three loci (corA, mgtA, and mgtB) exhibited no detectable Mg2+ uptake and required 10 mM Mg2+ in the medium for growth at the wild-type rate. A wild-type allele at any one of the three loci was sufficient to restore both Mg2+ transport and growth on 50 microM Mg2+. P22 transduction was used to map the mgt loci. The mgtA mutation was located to approximately 98 map units (cotransducible with pyrB), and mgtB mapped at about 80.5 map units (near gltC). A chromosomal library from S. typhimurium was screened for clones that complemented the Mg2+ requirement of a corA mgtA mgtB mutant. The three classes of plasmids obtained could each independently restore Mg2+ transport to this strain and corresponded to the corA, mgtA, and mgtB loci. Whereas the corA locus of S. typhimurium is analogous to the corA locus previously described for Escherichia coli, neither of the mgt loci described in this report appears analogous to the single mgt locus described in E. coli. Our data in this and the accompanying papers (M. D. Snavely, J. B. Florer, C. G. Miller, and M. E. Maguire, J. Bacteriol. 171:4752-4760, 4761-4766, 1989) indicate that the corA, mgtA, and mgtB loci of S. typhimurium represent three distinct systems that transport Mg2+.

Full text

PDF
4742

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bochner B. R., Savageau M. A. Generalized indicator plate for genetic, metabolic, and taxonomic studies with microorganisms. Appl Environ Microbiol. 1977 Feb;33(2):434–444. doi: 10.1128/aem.33.2.434-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chumley F. G., Menzel R., Roth J. R. Hfr formation directed by tn10. Genetics. 1979 Apr;91(4):639–655. doi: 10.1093/genetics/91.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grubbs R. D., Maguire M. E. Magnesium as a regulatory cation: criteria and evaluation. Magnesium. 1987;6(3):113–127. [PubMed] [Google Scholar]
  6. Gutterson N. I., Koshland D. E., Jr Replacement and amplification of bacterial genes with sequences altered in vitro. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4894–4898. doi: 10.1073/pnas.80.16.4894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hmiel S. P., Snavely M. D., Miller C. G., Maguire M. E. Magnesium transport in Salmonella typhimurium: characterization of magnesium influx and cloning of a transport gene. J Bacteriol. 1986 Dec;168(3):1444–1450. doi: 10.1128/jb.168.3.1444-1450.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hughes K. T., Roth J. R. Transitory cis complementation: a method for providing transposition functions to defective transposons. Genetics. 1988 May;119(1):9–12. doi: 10.1093/genetics/119.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  10. Kukral A. M., Strauch K. L., Maurer R. A., Miller C. G. Genetic analysis in Salmonella typhimurium with a small collection of randomly spaced insertions of transposon Tn10 delta 16 delta 17. J Bacteriol. 1987 May;169(5):1787–1793. doi: 10.1128/jb.169.5.1787-1793.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MARGOLIN P. Genetic fine structure of the leucine operon in Salmonella. Genetics. 1963 Mar;48:441–457. doi: 10.1093/genetics/48.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller C. G. Gentic mapping of Salmonella typhimurium peptidase mutations. J Bacteriol. 1975 Apr;122(1):171–176. doi: 10.1128/jb.122.1.171-176.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nelson D. L., Kennedy E. P. Magnesium transport in Escherichia coli. Inhibition by cobaltous ion. J Biol Chem. 1971 May 10;246(9):3042–3049. [PubMed] [Google Scholar]
  14. Nelson D. L., Kennedy E. P. Transport of magnesium by a repressible and a nonrepressible system in Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1091–1093. doi: 10.1073/pnas.69.5.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Park M. H., Wong B. B., Lusk J. E. Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology. J Bacteriol. 1976 Jun;126(3):1096–1103. doi: 10.1128/jb.126.3.1096-1103.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sanderson K. E., Roth J. R. Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev. 1988 Dec;52(4):485–532. doi: 10.1128/mr.52.4.485-532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Silver S. Active transport of magnesium in escherichia coli. Proc Natl Acad Sci U S A. 1969 Mar;62(3):764–771. doi: 10.1073/pnas.62.3.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silver S., Johnseine P., Whitney E., Clark D. Manganese-resistant mutants of Escherichia coli: physiological and genetic studies. J Bacteriol. 1972 Apr;110(1):186–195. doi: 10.1128/jb.110.1.186-195.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Snavely M. D., Florer J. B., Miller C. G., Maguire M. E. Magnesium transport in Salmonella typhimurium: 28Mg2+ transport by the CorA, MgtA, and MgtB systems. J Bacteriol. 1989 Sep;171(9):4761–4766. doi: 10.1128/jb.171.9.4761-4766.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Snavely M. D., Florer J. B., Miller C. G., Maguire M. E. Magnesium transport in Salmonella typhimurium: expression of cloned genes for three distinct Mg2+ transport systems. J Bacteriol. 1989 Sep;171(9):4752–4760. doi: 10.1128/jb.171.9.4752-4760.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walker G. M. Magnesium and cell cycle control: an update. Magnesium. 1986;5(1):9–23. [PubMed] [Google Scholar]
  22. Way J. C., Davis M. A., Morisato D., Roberts D. E., Kleckner N. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene. 1984 Dec;32(3):369–379. doi: 10.1016/0378-1119(84)90012-x. [DOI] [PubMed] [Google Scholar]
  23. Webb M. Interrelationships between the utilization of magnesium and the uptake of other bivalent cations by bacteria. Biochim Biophys Acta. 1970 Nov 24;222(2):428–439. doi: 10.1016/0304-4165(70)90133-9. [DOI] [PubMed] [Google Scholar]
  24. Whitfield H. J., Levine G. Isolation and characterization of a mutant of Salmonella typhimurium deficient in a major deoxyribonucleic acid polymerase activity. J Bacteriol. 1973 Oct;116(1):54–58. doi: 10.1128/jb.116.1.54-58.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES