Abstract
Wild-type cultures of Scytalidium flavo-brunneum produce a 15-azasterol antifungal agent and a reddish brown pigment as secondary metabolites. Spontaneous mutants of S. flavo-brunneum that had simultaneously lost the ability to produce the 15-azasterol and the pigment were transformed with plasmid pSFB-1, which was obtained from wild-type S. flavo-brunneum. Each transformant possessed the plasmid and coincidentally reacquired azasterol and pigment production. Regulation of transcription and relative plasmid copy number was determined as a function of the culture cycle of the organism. Twenty-five-fold amplification of the plasmid was observed in the fungus during the stationary phase. RNA transcripts of 0.9, 1.0, 1.5, 2.0, and 2.5 kilobases were expressed from the plasmid by the organism. While differences in the temporal regulation of the transcripts were seen, all except the 1.0-kilobase transcript increased in abundance on entry of the culture into the stationary phase of growth.
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- Bailey R. B., Hays P. R., Parks L. W. Homoazasterol-mediated inhibition of yeast sterol biosynthesis. J Bacteriol. 1976 Dec;128(3):730–734. doi: 10.1128/jb.128.3.730-734.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boeck L. D., Hoehn M. M., Westhead J. E., Wolter R. K., Thomas D. N. New azasteroidal antifungal antibotics from Geotrichum flavo-brunneum. I. Discovery and fermentation studies. J Antibiot (Tokyo) 1975 Feb;28(2):95–101. doi: 10.7164/antibiotics.28.95. [DOI] [PubMed] [Google Scholar]
- Caprioglio D. R., Parks L. W. Purification and characterization of plasmid-like DNA from the antimycotic producing fungus, Scytalidium flavo-brunneum. Plasmid. 1988 Nov;20(3):175–181. doi: 10.1016/0147-619x(88)90024-8. [DOI] [PubMed] [Google Scholar]
- Garber R. C., Yoder O. C. Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components. Anal Biochem. 1983 Dec;135(2):416–422. doi: 10.1016/0003-2697(83)90704-2. [DOI] [PubMed] [Google Scholar]
- Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
- Rodriguez R. J., Parks L. W. Growth and antifungal homoazasterol production in Geotrichum flavo-brunneum. Antimicrob Agents Chemother. 1980 Nov;18(5):822–828. doi: 10.1128/aac.18.5.822. [DOI] [PMC free article] [PubMed] [Google Scholar]