Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Sep;171(9):5183–5186. doi: 10.1128/jb.171.9.5183-5186.1989

Levels of DNA topoisomerases, single-stranded-DNA-binding protein, and DNA polymerase I in rho+ and rho-15 strains of Escherichia coli.

G F Arnold 1, T A Phillips 1, I Tessman 1
PMCID: PMC210337  PMID: 2549016

Abstract

The Escherichia coli rho-15 mutant, which is highly defective in transcription termination, was examined to see whether its reduced DNA superhelicity could be explained by altered expression of proteins that may affect DNA structure. Levels of DNA gyrase and topoisomerase I were normal; levels of single-stranded-DNA-binding protein, DNA polymerase I, and a protein tentatively identified as Lon were significantly altered.

Full text

PDF
5183

Images in this article

5184Image
on p.5184

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold G. F., Tessman I. Regulation of DNA superhelicity by rpoB mutations that suppress defective Rho-mediated transcription termination in Escherichia coli. J Bacteriol. 1988 Sep;170(9):4266–4271. doi: 10.1128/jb.170.9.4266-4271.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumberg S., Lovett M. G. Reduced recovery of plasmid transconjugants in crosses with Escherichia coli rho mutant recipients. Plasmid. 1977 Nov;1(1):118–122. doi: 10.1016/0147-619x(77)90014-2. [DOI] [PubMed] [Google Scholar]
  3. Beckmann J. S., Tichauer Y., Daniel V., Littauer U. Z. Binding of the termination factor rho to DNA. Biochem Biophys Res Commun. 1971 May 21;43(4):806–813. doi: 10.1016/0006-291x(71)90688-7. [DOI] [PubMed] [Google Scholar]
  4. Chase J. W., Williams K. R. Single-stranded DNA binding proteins required for DNA replication. Annu Rev Biochem. 1986;55:103–136. doi: 10.1146/annurev.bi.55.070186.000535. [DOI] [PubMed] [Google Scholar]
  5. Das A., Court D., Adhya S. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959–1963. doi: 10.1073/pnas.73.6.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiNardo S., Voelkel K. A., Sternglanz R., Reynolds A. E., Wright A. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell. 1982 Nov;31(1):43–51. doi: 10.1016/0092-8674(82)90403-2. [DOI] [PubMed] [Google Scholar]
  7. Fassler J. S., Arnold G. F., Tessman I. Reduced superhelicity of plasmid DNA produced by the rho-15 mutation in Escherichia coli. Mol Gen Genet. 1986 Sep;204(3):424–429. doi: 10.1007/BF00331019. [DOI] [PubMed] [Google Scholar]
  8. Fassler J. S., Tessman I. Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants. J Virol. 1981 Mar;37(3):955–962. doi: 10.1128/jvi.37.3.955-962.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fassler J. S., Tessman I., Tessman E. S. Lethality of the double mutations rho rep and rho ssb in Escherichia coli. J Bacteriol. 1985 Feb;161(2):609–614. doi: 10.1128/jb.161.2.609-614.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Funnell B. E., Baker T. A., Kornberg A. Complete enzymatic replication of plasmids containing the origin of the Escherichia coli chromosome. J Biol Chem. 1986 Apr 25;261(12):5616–5624. [PubMed] [Google Scholar]
  11. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  12. Light J., Baumberg S. Further studies on the inviability of Escherichia coli K12 rho mutant strains carrying IncFII plasmids. Mol Gen Genet. 1983;189(2):309–313. doi: 10.1007/BF00337822. [DOI] [PubMed] [Google Scholar]
  13. Menzel R., Gellert M. Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling. Cell. 1983 Aug;34(1):105–113. doi: 10.1016/0092-8674(83)90140-x. [DOI] [PubMed] [Google Scholar]
  14. Neidhardt F. C., Vaughn V., Phillips T. A., Bloch P. L. Gene-protein index of Escherichia coli K-12. Microbiol Rev. 1983 Jun;47(2):231–284. doi: 10.1128/mr.47.2.231-284.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  16. Okazaki R., Arisawa M., Sugino A. Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954–2957. doi: 10.1073/pnas.68.12.2954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Olivera B. M., Lehman I. R. Enzymic joining of polynucleotides. 3. The polydeoxyadenylate-polydeoxythymidylate homopolymer pair. J Mol Biol. 1968 Sep 14;36(2):261–274. doi: 10.1016/0022-2836(68)90380-x. [DOI] [PubMed] [Google Scholar]
  18. Orr E., Staudenbauer W. L. An Escherichia coli mutant thermosensitive in the B subunit of DNA gyrase: effect on the structure and replication of the colicin E1 plasmid in vitro. Mol Gen Genet. 1981;181(1):52–56. doi: 10.1007/BF00339004. [DOI] [PubMed] [Google Scholar]
  19. Pedersen S., Reeh S. V. Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages lambdadrifd 18 and lambdadfus-3. Mol Gen Genet. 1976 Mar 30;144(3):339–343. doi: 10.1007/BF00341733. [DOI] [PubMed] [Google Scholar]
  20. Pruss G. J., Manes S. H., Drlica K. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes. Cell. 1982 Nov;31(1):35–42. doi: 10.1016/0092-8674(82)90402-0. [DOI] [PubMed] [Google Scholar]
  21. Richardson J. P., Grimley C., Lowery C. Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations. Proc Natl Acad Sci U S A. 1975 May;72(5):1725–1728. doi: 10.1073/pnas.72.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Simon L. D., Gottesman M., Tomczak K., Gottesman S. Hyperdegradation of proteins in Escherichia coli rho mutants. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1623–1627. doi: 10.1073/pnas.76.4.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steck T. R., Pruss G. J., Manes S. H., Burg L., Drlica K. DNA supercoiling in gyrase mutants. J Bacteriol. 1984 May;158(2):397–403. doi: 10.1128/jb.158.2.397-403.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taylor D. E., Levine J. G. Characterization of a plasmid mutation affecting maintenance, transfer and elimination by novobiocin. Mol Gen Genet. 1979 Jul 13;174(2):127–133. doi: 10.1007/BF00268350. [DOI] [PubMed] [Google Scholar]
  25. Tessman I., Fassler J. S., Bennett D. C. Relative map location of the rep and rho genes of Escherichia coli. J Bacteriol. 1982 Sep;151(3):1637–1640. doi: 10.1128/jb.151.3.1637-1640.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tse-Dinh Y. C. Regulation of the Escherichia coli DNA topoisomerase I gene by DNA supercoiling. Nucleic Acids Res. 1985 Jul 11;13(13):4751–4763. doi: 10.1093/nar/13.13.4751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wanner B. L., Kodaira R., Neidhardt F. C. Physiological regulation of a decontrolled lac operon. J Bacteriol. 1977 Apr;130(1):212–222. doi: 10.1128/jb.130.1.212-222.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wolfson J. S., Hooper D. C., Swartz M. N., McHugh G. L. Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMG110 from Escherichia coli. J Bacteriol. 1982 Oct;152(1):338–344. doi: 10.1128/jb.152.1.338-344.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. von Wright A., Bridges B. A. Effect of gyrB-mediated changes in chromosome structure on killing of Escherichia coli by ultraviolet light: experiments with strains differing in deoxyribonucleic acid repair capacity. J Bacteriol. 1981 Apr;146(1):18–23. doi: 10.1128/jb.146.1.18-23.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES