Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Oct;171(10):5405–5409. doi: 10.1128/jb.171.10.5405-5409.1989

Chromosome partition in Escherichia coli requires postreplication protein synthesis.

W D Donachie 1, K J Begg 1
PMCID: PMC210377  PMID: 2676975

Abstract

After inhibition of protein synthesis, the number of nuclear bodies (nucleoids) visible in cells of Escherichia coli B/rA corresponded closely to the number of completely replicated chromosomes. We calculated that nucleoid partition follows almost immediately after replication forks reach the chromosome terminus. We show that such a partition is dependent on protein synthesis and that this may reflect the requirement that cells must achieve a certain minimum length before partition (and subsequent cell division) can take place.

Full text

PDF
5405

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  2. Donachie W. D., Begg K. J. Cell length, nucleoid separation, and cell division of rod-shaped and spherical cells of Escherichia coli. J Bacteriol. 1989 Sep;171(9):4633–4639. doi: 10.1128/jb.171.9.4633-4639.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donachie W. D., Begg K. J., Vicente M. Cell length, cell growth and cell division. Nature. 1976 Nov 25;264(5584):328–333. doi: 10.1038/264328a0. [DOI] [PubMed] [Google Scholar]
  4. Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
  5. Hussain K., Begg K. J., Salmond G. P., Donachie W. D. ParD: a new gene coding for a protein required for chromosome partitioning and septum localization in Escherichia coli. Mol Microbiol. 1987 Jul;1(1):73–81. doi: 10.1111/j.1365-2958.1987.tb00529.x. [DOI] [PubMed] [Google Scholar]
  6. Kubitschek H. E. Estimation of the D period from residual division after exposure of exponential phase bacteria to chloramphenicol. Mol Gen Genet. 1974;135(2):123–130. doi: 10.1007/BF00264780. [DOI] [PubMed] [Google Scholar]
  7. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  8. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  9. POWELL E. O. Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol. 1956 Dec;15(3):492–511. doi: 10.1099/00221287-15-3-492. [DOI] [PubMed] [Google Scholar]
  10. SCHAECHTER M. Patterns of cellular control during unbalanced growth. Cold Spring Harb Symp Quant Biol. 1961;26:53–62. doi: 10.1101/sqb.1961.026.01.011. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES