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. 1989 Oct;171(10):5753–5755. doi: 10.1128/jb.171.10.5753-5755.1989

A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli.

D Spikes 1, J K Setlow 1
PMCID: PMC210427  PMID: 2676992

Abstract

The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain. In H. influenzae, pMucAMucB caused a small but reproducible increase in survival after UV irradiation in wild-type cells and in a rec-1 mutant, but there was no increase in spontaneous mutation in the wild type or in the rec-1 mutant and no UV-induced mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balganesh M., Setlow J. K. Genes from plasmid pKM101 in Haemophilus influenzae: separation of functions of mucA and mucB. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7753–7756. doi: 10.1073/pnas.82.22.7753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown A. M., Willetts N. S. A physical and genetic map of the IncN plasmid R46. Plasmid. 1981 Mar;5(2):188–201. doi: 10.1016/0147-619x(81)90020-2. [DOI] [PubMed] [Google Scholar]
  3. Elledge S. J., Walker G. C. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. J Mol Biol. 1983 Feb 25;164(2):175–192. doi: 10.1016/0022-2836(83)90074-8. [DOI] [PubMed] [Google Scholar]
  4. Kimball R. F., Boling M. E., Perdue S. W. Evidence that UV-inducible error-prone repair is absent in Haemophilus influenzae Rd, with a discussion of the relation to error-prone repair of alkylating-agent damage. Mutat Res. 1977 Aug;44(2):183–196. doi: 10.1016/0027-5107(77)90076-8. [DOI] [PubMed] [Google Scholar]
  5. McCarthy D., Clayton N. L., Setlow J. K. A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli. J Bacteriol. 1982 Sep;151(3):1605–1607. doi: 10.1128/jb.151.3.1605-1607.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nohmi T., Battista J. R., Dodson L. A., Walker G. C. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1816–1820. doi: 10.1073/pnas.85.6.1816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Notani N. K., Setlow J. K. Inducible repair system in Haemophilus influenzae unaccompanied by mutation. J Bacteriol. 1980 Jul;143(1):516–519. doi: 10.1128/jb.143.1.516-519.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Notani N. K., Setlow J. K., McCarthy D., Clayton N. L. Transformation of Haemophilus influenzae by plasmid RSF0885. J Bacteriol. 1981 Dec;148(3):812–816. doi: 10.1128/jb.148.3.812-816.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Perry K. L., Elledge S. J., Mitchell B. B., Marsh L., Walker G. C. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4331–4335. doi: 10.1073/pnas.82.13.4331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Perry K. L., Walker G. C. Identification of plasmid (pKM101)-coded proteins involved in mutagenesis and UV resistance. Nature. 1982 Nov 18;300(5889):278–281. doi: 10.1038/300278a0. [DOI] [PubMed] [Google Scholar]
  11. Setlow J. K., Boling M. E., Allison D. P., Beattie K. L. Relationship between prophage induction and transformation in Haemophilus influenzae. J Bacteriol. 1973 Jul;115(1):153–161. doi: 10.1128/jb.115.1.153-161.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Setlow J. K., Spikes D., Griffin K. Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli. J Bacteriol. 1988 Sep;170(9):3876–3881. doi: 10.1128/jb.170.9.3876-3881.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shanabruch W. G., Walker G. C. Localization of the plasmid (pKM101) gene(s) involved in recA+lexA+-dependent mutagenesis. Mol Gen Genet. 1980;179(2):289–297. doi: 10.1007/BF00425456. [DOI] [PubMed] [Google Scholar]
  14. Shinagawa H., Kato T., Ise T., Makino K., Nakata A. Cloning and characterization of the umu operon responsible for inducible mutagenesis in Escherichia coli. Gene. 1983 Aug;23(2):167–174. doi: 10.1016/0378-1119(83)90048-3. [DOI] [PubMed] [Google Scholar]
  15. Steinhart W. L., Herriott R. M. Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae. J Bacteriol. 1968 Nov;96(5):1718–1724. doi: 10.1128/jb.96.5.1718-1724.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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