Abstract
Methylation by Ava methylases in Escherichia coli increases the efficiency to transfer of Tn5 in pBR322bla:: Tn5 from E. coli to Anabaena sp. strain PCC 7120 by conjugation. Following conjugation, Tn5 but not pBR322 sequences were found at many different positions in the Anabaena chromosome. This procedure was used to mutagenize, tag, and clone a previously unrecognized gene required for nitrogen fixation in this Anabaena sp.
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- Currier T. C., Haury J. F., Wolk C. P. Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium. J Bacteriol. 1977 Mar;129(3):1556–1562. doi: 10.1128/jb.129.3.1556-1562.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elhai J., Wolk C. P. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 1988;167:747–754. doi: 10.1016/0076-6879(88)67086-8. [DOI] [PubMed] [Google Scholar]
- Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene. 1982 Jun;18(3):289–296. doi: 10.1016/0378-1119(82)90167-6. [DOI] [PubMed] [Google Scholar]
- Golden J. W., Wiest D. R. Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science. 1988 Dec 9;242(4884):1421–1423. doi: 10.1126/science.3144039. [DOI] [PubMed] [Google Scholar]
- Grillo J. F., Bottomley P. J., Van Baalen C., Tabita F. R. A mutant of Anabaena sp. CA with oxygen-sensitive nitrogenase activity. Biochem Biophys Res Commun. 1979 Jul 27;89(2):685–693. doi: 10.1016/0006-291x(79)90684-3. [DOI] [PubMed] [Google Scholar]
- Haury J. F., Wolk C. P. Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity. J Bacteriol. 1978 Nov;136(2):688–692. doi: 10.1128/jb.136.2.688-692.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
- Rice D., Mazur B. J., Haselkorn R. Isolation and physical mapping of nitrogen fixation genes from the cyanobacterium Anabaena 7120. J Biol Chem. 1982 Nov 10;257(21):13157–13163. [PubMed] [Google Scholar]
- Shapira S. K., Chou J., Richaud F. V., Casadaban M. J. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase. Gene. 1983 Nov;25(1):71–82. doi: 10.1016/0378-1119(83)90169-5. [DOI] [PubMed] [Google Scholar]
- Wilcox M., Mitchison G. J., Smith R. J. Mutants of Anabaena cylindrica altered in heterocyst spacing. Arch Microbiol. 1975 May 5;103(3):219–223. doi: 10.1007/BF00436353. [DOI] [PubMed] [Google Scholar]
- Wolk C. P., Cai Y., Cardemil L., Flores E., Hohn B., Murry M., Schmetterer G., Schrautemeier B., Wilson R. Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen. J Bacteriol. 1988 Mar;170(3):1239–1244. doi: 10.1128/jb.170.3.1239-1244.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolk C. P., Vonshak A., Kehoe P., Elhai J. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1561–1565. doi: 10.1073/pnas.81.5.1561. [DOI] [PMC free article] [PubMed] [Google Scholar]