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. 1989 Nov;171(11):6039–6042. doi: 10.1128/jb.171.11.6039-6042.1989

Purification and some properties of glutathione S-transferase from Escherichia coli B.

M Iizuka 1, Y Inoue 1, K Murata 1, A Kimura 1
PMCID: PMC210469  PMID: 2553668

Abstract

Glutathione S-transferase was purified approximately 2,300-fold from cell extracts of Escherichia coli B with a 7.5% activity yield. The molecular weight of the enzyme was 45,000, and the enzyme appeared to consist of two homogeneous subunits. The enzyme was almost specific to 1-chloro-2,4-dinitrobenzene (Km, 1.43 mM) and glutathione (Km, 0.33 mM). The optimal pH and optimal temperature for activity were 7.0 and 50 degrees C, respectively, and the enzyme was stable from pH 5 to 11. The activity of the enzyme for 1-chloro-2,4-dinitrobenzene (3,2 mumol/min per mg of protein) was significantly lower than those of the enzymes from mammals, plants, and fungi.

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Selected References

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