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. 1989 Nov;171(11):6259–6264. doi: 10.1128/jb.171.11.6259-6264.1989

Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae.

F Klebl 1, W Tanner 1
PMCID: PMC210497  PMID: 2509432

Abstract

A major protein of Saccharomyces cerevisiae cell walls is a 29-kilodalton glycoprotein which shows lectinlike binding to beta-1,3-glucan and chitin. It was solubilized by heating isolated cell walls at 90 degrees C and purified to homogeneity by running two high-pressure liquid chromatography columns. With the sequence information of the N terminus and seven peptides, two oligonucleotides were synthesized and the gene was cloned. Its sequence is similar to those of two plant beta-glucanases, and the protein was shown to possess beta-1,3-exoglucanase activity with laminarin as substrate. Haploid yeast cells contained one copy of the gene (BGL2). Gene disruption did not result in a phenotype.

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Selected References

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