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. 1989 Nov;171(11):6372–6374. doi: 10.1128/jb.171.11.6372-6374.1989

Purification and analysis of colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae from enterotoxigenic Escherichia coli.

R H Hall 1, D R Maneval Jr 1, J H Collins 1, J L Theibert 1, M M Levine 1
PMCID: PMC210515  PMID: 2572583

Abstract

Enterotoxigenic Escherichia coli fimbriae are immunogenic and play a key role in intestinal colonization. Native colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae were purified by a common method involving shearing, differential centrifugation, gel filtration, and density gradient ultracentrifugation. The compositions and N-terminal sequences were determined. Coli surface antigen 3 possesses two N-terminal isoforms, one of which matches the published DNA sequence, except for the previously proposed signal sequence cleavage point.

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Selected References

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