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. 1989 Dec;171(12):6776–6781. doi: 10.1128/jb.171.12.6776-6781.1989

Isolation and characterization of a Saccharomyces cerevisiae mutant with impaired glutamate synthase activity.

J L Folch 1, A Antaramián 1, L Rodríguez 1, A Bravo 1, A Brunner 1, A González 1
PMCID: PMC210576  PMID: 2687252

Abstract

A mutant of Saccharomyces cerevisiae that lacks glutamate synthase (GOGAT) activity has been isolated. This mutant was obtained after chemical mutagenesis of a NADP-glutamate dehydrogenase-less mutant strain. The gdh gus mutant is a glutamate auxotroph. The genetic analysis of the gus mutant showed that the GOGAT-less phenotype is due to the presence of two loosely linked mutations. Evidence is presented which suggests the possibility that S. cerevisiae has two GOGAT activities, designated GOGAT A and GOGAT B. These activities can be distinguished by their pH optima and by their regulation by glutamate. Furthermore, one of the mutations responsible for the GOGAT-less phenotype affected GOGAT A activity, while the other mutation affected GOGAT B activity.

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Selected References

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