Abstract
Corpora lutea from the period of delayed implantation and from early postimplantation stages of the armadillo, mink, and rat were fixed in buffered osmium tetroxide-sucrose or potassium permanganate. After rapid dehydration, the portions of the corpora lutea were embedded in either methacrylate or epoxy resin. Examination of the lutein cells by electron microscopy revealed the presence, in the better preserved material, of an extensive development of tubular agranular endoplasmic reticulum. Although the membranes of the endoplasmic reticulum are the most striking feature of the lutein cells of both stages of the three animals examined, very numerous large mitochondria with cristae that exhibit a variety of forms tending toward villiform, and protrusions and foldings of the lutein cell margins on the pericapillary space are also characteristic of these cells. Certain minor differences in the lutein cells of the species examined are also noted. No indications of conversion of mitochondria into lipid, of accumulation of lipid in the Golgi area, or of the protrusion of lutein cells into spaces between the endothelial cells, as suggested by other authors, were noted in these preparations. Some of the difficulties inherent in the visualization of the secretory activity of cells producing steroid hormones are briefly discussed.
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- BELT W. D., PEASE D. C. Mitochondrial structure in sites of steroid secretion. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):369–374. [PMC free article] [PubMed] [Google Scholar]
- CHRISTENSEN A. K., CHAPMAN G. B. Cup-shaped mitochondria in interstitial cells of the albino rat testis. Exp Cell Res. 1959 Nov;18:576–579. doi: 10.1016/0014-4827(59)90323-4. [DOI] [PubMed] [Google Scholar]
- CHRISTENSEN A. K., FAWCETT D. W. The normal fine structure of opossum testicular interstitial cells. J Biophys Biochem Cytol. 1961 Mar;9:653–670. doi: 10.1083/jcb.9.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALTON A. J., ZEIGEL R. F. A simplified method of staining thin sections of biolgical material with lead hydroxide for electron microscopy. J Biophys Biochem Cytol. 1960 Apr;7:409–410. doi: 10.1083/jcb.7.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAWSON A. B., VELARDO J. T. A histochemical study of lipids of the corpora lutea of the rat during pseudopregnancy. Am J Anat. 1955 Sep;97(2):303–329. doi: 10.1002/aja.1000970205. [DOI] [PubMed] [Google Scholar]
- DEANE H. W. Histochemical observations on the ovary and oviduct of the albino rat during the estrous cycle. Am J Anat. 1952 Nov;91(3):363–413. doi: 10.1002/aja.1000910303. [DOI] [PubMed] [Google Scholar]
- DORFMAN R. I., HAYANO M., HECHTER O., SABA N. Some aspects of the biogenesis of adrenal steroid hormones. Recent Prog Horm Res. 1956;12:79-118; discussion, 118-23. [PubMed] [Google Scholar]
- KARNOVSKY M. L., DEANE H. W. Aldehyde formation in the lipide droplets of the adrenal cortex during fixation, as demonstrated chemically and histochemically. J Histochem Cytochem. 1955 Mar;3(2):85–102. doi: 10.1177/3.2.85. [DOI] [PubMed] [Google Scholar]
- LEVER J. D. Physiologically induced changes in adrenocortical mitochondria. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):313–318. doi: 10.1083/jcb.2.4.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVER J. D. Remarks on the electron microscopy of the rat luteum and comparison with earlier observations on the adrenal cortex. Anat Rec. 1956 Jan;124(1):111–125. doi: 10.1002/ar.1091240109. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MERRIAM R. W. The contribution of lower oxides of osmium to the density of biological specimens in electron microscopy. J Biophys Biochem Cytol. 1958 Sep 25;4(5):579–582. doi: 10.1083/jcb.4.5.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PORTER K. R., YAMADA E. Studies on the endoplasmic reticulum. V. Its form and differentiation in pigment epithelial cells of the frog retina. J Biophys Biochem Cytol. 1960 Sep;8:181–205. doi: 10.1083/jcb.8.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSS M. H., PAPPAS G. D., LANMAN J. T., LIND J. Electron microscope observations on the endoplasmic reticulum in the human fetal adrenal. J Biophys Biochem Cytol. 1958 Sep 25;4(5):659–661. doi: 10.1083/jcb.4.5.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SABATINI D. D., DE ROBERTIS E. D. Ultrastructural zonation of adrenocortex in the rat. J Biophys Biochem Cytol. 1961 Jan;9:105–119. doi: 10.1083/jcb.9.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Further observations on the nuclear envelope of the animal cell. J Biophys Biochem Cytol. 1959 Oct;6:147–156. doi: 10.1083/jcb.6.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]