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. 1962 Jan 1;12(1):1–15. doi: 10.1083/jcb.12.1.1

A CYTOCHEMICAL DESCRIPTION OF THE MULTIPLICATION OF MENGOVIRUS IN L-929 CELLS

Richard M Franklin 1
PMCID: PMC2106010  PMID: 13894687

Abstract

A correlation of cytochemical changes with virus production has been studied in L cells infected with Mengovirus. After a latent period of about 2 hours, virus was produced rapidly, reaching maximum titers of up to 12,000 particles per cell in 6 to 8 hours. The earliest cytological change was in the nucleus and consisted of a slight condensation of chromatin. There is no evidence, however, for the multiplication of either the viral RNA or protein in the nucleus. RNA, of high molecular weight, accumulated in the perinuclear area of the cytoplasm and was later found in inclusions. The perinuclear RNA was digestible with RNase and may be located in or on ribosomes. The inclusion RNA was resistant to RNase but could be removed by pepsin or potassium permanganate; it is probably in completed virus particles. Viral antigen was first observed in a perinuclear location and later in the above-mentioned inclusions. Although the viral protein contains appreciable amounts of arginine and lysine, it is not a basic protein of the histone type. Phase-contrast microscopy of living cells clearly demonstrated the role of the inclusions in release of virus from infected cells. A comparison is made between these cytological changes in Mengo-infected cells and those which have been found by other workers in polio-infected cells. There are many very similar changes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOCH D. P., HEW H. Y. Schedule of spermatogenesis in the pulmonate snail Helix aspersa, with special reference to histone transition. J Biophys Biochem Cytol. 1960 Jun;7:515–532. doi: 10.1083/jcb.7.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COONS A. H. Fluorescent antibody methods. Gen Cytochem Methods. 1958;1:399–422. [PubMed] [Google Scholar]
  3. DICK G. W. A. The relationship of Mengo encephalomyelitis, encephalomyocarditis, Columbia-SK and M.M. viruses. J Immunol. 1949 Aug;62(4):375–386. [PubMed] [Google Scholar]
  4. DULBECCO R. A consideration of virus-host relationship in virus-induced neoplasia at the cellular level. Cancer Res. 1960 Jun;20:751–761. [PubMed] [Google Scholar]
  5. DUNNEBACKE T. H. Correlation of the stage of cytopathic change with the release of poliomyelitis virus. Virology. 1956 Jun;2(3):399–410. doi: 10.1016/0042-6822(56)90034-4. [DOI] [PubMed] [Google Scholar]
  6. DUNNEBACKE T. H. Cytopathic changes associated with poliomyelitis infections in human amnion cells. Virology. 1956 Dec;2(6):811–819. doi: 10.1016/0042-6822(56)90060-5. [DOI] [PubMed] [Google Scholar]
  7. FRANKLIN R. M. Comparison of assays for Mengovirus and Reovirus-3. Proc Soc Exp Biol Med. 1961 Jul;107:651–653. doi: 10.3181/00379727-107-26716. [DOI] [PubMed] [Google Scholar]
  8. FRANKLIN R. M., HENRY C. The multiplication of fowl plague virus in tissue cultures of chick embryo cells. Virology. 1960 Apr;10:406–418. doi: 10.1016/0042-6822(60)90125-2. [DOI] [PubMed] [Google Scholar]
  9. GRANOFF A. Noninfectious forms of Newcastle disease and influenza viruses; studies on noninfectious virus occurring within cells that are producing fully infectious virus. Virology. 1955 Dec;1(5):516–532. doi: 10.1016/0042-6822(55)90040-4. [DOI] [PubMed] [Google Scholar]
  10. HOWES D. W., MELNICK J. L., REISSIG M. Sequence of morphological changes in epithelial cell cultures infected with poliovirus. J Exp Med. 1956 Sep 1;104(3):289–304. doi: 10.1084/jem.104.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LWOFF A., DULBECCO R., VOGT M., LWOFF M. Kinetics of the release of poliomyelitis virus from single cells. Virology. 1955 May;1(1):128–139. doi: 10.1016/0042-6822(55)90010-6. [DOI] [PubMed] [Google Scholar]
  13. REICH E., FRANKLIN R. M. Effect of mitomycin C on the growth of some animal viruses. Proc Natl Acad Sci U S A. 1961 Aug;47:1212–1217. doi: 10.1073/pnas.47.8.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUM E. L. Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science. 1961 Aug 25;134(3478):556–557. doi: 10.1126/science.134.3478.556. [DOI] [PubMed] [Google Scholar]
  15. SHALLA T. A. Degradation of tobacco mosaic virus by potassium permanganate. Virology. 1961 Mar;13:383–386. doi: 10.1016/0042-6822(61)90166-0. [DOI] [PubMed] [Google Scholar]
  16. SMITH K. C. Isolation of "soluble RNA" without an ultracentrifuge. Biochim Biophys Acta. 1960 May 20;40:360–361. doi: 10.1016/0006-3002(60)91366-4. [DOI] [PubMed] [Google Scholar]
  17. TENENBAUM E. Changes in cellular nucleic acids during infection with poliomyelitis virus as studied by fluorescence microscopy. Nature. 1957 Nov 16;180(4594):1044–1044. doi: 10.1038/1801044a0. [DOI] [PubMed] [Google Scholar]
  18. WARREN J., SMADEL J. E., RUSS S. B. The family relationship of encephalomyocarditis, Columbia-SK, M.M., and Mengo encephalomyelitis viruses. J Immunol. 1949 Aug;62(4):387–398. [PubMed] [Google Scholar]

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