Abstract
After intravenous injection of horseradish peroxidase into rats, the foreign protein appeared in the kidney first in the small phagosomes and its concentration there decreased quickly; it then was concentrated and "stored" for several days in the large phagosomes. After injection of 10 mg of peroxidase per 100 gm of body weight, the concentration of peroxidase in blood and urine decreased exponentially during the first 6 hours; small amounts of peroxidase were excreted in the urine for several days. When 0.05 to 1.0 mg of peroxidase per 100 gm were administered, most of the peroxidase was taken up by the liver and little by the kidney, and a portion was excreted in the urine even at the lowest dose. At doses above 1.5 mg per 100 gm, the liver cells were saturated, and large reabsorption droplets appeared in the tubule cells of the kidney. With further dosage increase, the concentration of peroxidase in the phagosomes of the kidney increased rapidly until saturation was reached at doses of 13 mg per 100 gm. After intraperitoneal injection of egg white 18 hours prior to the administration of peroxidase, the concentration of peroxidase in all kidney fractions was only 10 to 25 per cent of the values for the untreated animals, the disappearance of peroxidase from the blood was delayed, and 81 percent more peroxidase was excreted in the urine. The treatment with egg white had no effect on the uptake of peroxidase by the liver. The ability of kidney tissue to degrade and adsorb peroxidase in vitro was tested.
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Selected References
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- COHN Z. A., HIRSCH J. G. The influence of phagocytosis on the intracellular distribution of granule-associated components of polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:1015–1022. doi: 10.1084/jem.112.6.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A., HIRSCH J. G. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:983–1004. doi: 10.1084/jem.112.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALCQ A. M. [New constituents of the egg of Ascidia revealed by metachromatic vital dyes]. Arch Biol (Liege) 1960 Jan-Mar;71:93–139. [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FARQUHAR M. G., PALADE G. E. Segregation of ferritin in glomerular protein absorption droplets. J Biophys Biochem Cytol. 1960 Apr;7:297–304. doi: 10.1083/jcb.7.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HIRSCH J. G., COHN Z. A. Degranulation of polymorphonuclear leucocytes following phagocytosis of microorganisms. J Exp Med. 1960 Dec 1;112:1005–1014. doi: 10.1084/jem.112.6.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLTER H. Pinocytosis. Int Rev Cytol. 1959;8:481–504. doi: 10.1016/s0074-7696(08)62738-2. [DOI] [PubMed] [Google Scholar]
- OLIVER J., MACDOWELL M., LEE Y. C. Cellular mechanisms of protein metabolism in the nephron. I. The structural aspects of proteinuria; tubular absorption, droplet formation, and the disposal of proteins. J Exp Med. 1954 Jun 1;99(6):589–604. doi: 10.1084/jem.99.6.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- QUERTIER J., BRACHET J. [Study of the distribution of metachromatic vacuoles and of acid phosphatase in the amoeba Amoeba proteus]. Arch Biol (Liege) 1959 Apr-Jun;70:153–167. [PubMed] [Google Scholar]
- STRAUS W. Colorimetric determination of cytochrome c oxidase by formation of a quinoedimonium pigment from dimethyl-p-phenylenediamine. Biochim Biophys Acta. 1956 Jan;19(1):58–65. doi: 10.1016/0006-3002(56)90385-7. [DOI] [PubMed] [Google Scholar]
- STRAUS W. Concentration of acid phosphatase, ribonuclease, desoxyribonuclease, beta-glucuronidase, and cathepsin in droplets isolated from the kidney cells of normal rats. J Biophys Biochem Cytol. 1956 Sep 25;2(5):513–521. doi: 10.1083/jcb.2.5.513. [DOI] [PMC free article] [PubMed] [Google Scholar]