Abstract
Electron microscopic observations on chondrocytes from rabbit ear cartilage 72 hours after papain has been given intravenously show that many vacuoles in the Golgi apparatus contain a material with a speckled appearance. Some Golgi vacuoles also contain a material with a banded pattern which can be identified as a collagen. The significance of this observation is discussed.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARGMANN W., KNOOP A. Uber die Morphologie der Milchsekretion; lichtund elektronenmikroskopische Studien an der Milchdrüse Ratte. Z Zellforsch Mikrosk Anat. 1959;49(3):344–388. [PubMed] [Google Scholar]
- BURGOS M. H., FAWCETT D. W. Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol. 1955 Jul 25;1(4):287–300. doi: 10.1083/jcb.1.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAMERON D. A. The fine structure of osteoblasts in the metaphysis of the tibia of the young rat. J Biophys Biochem Cytol. 1961 Mar;9:583–595. doi: 10.1083/jcb.9.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARO L. G. Electron microscopic radioautography of thin sections: the Golgi zone as a site of protein concentration in pancreatic acinar cells. J Biophys Biochem Cytol. 1961 May;10:37–45. doi: 10.1083/jcb.10.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHAPMAN J. A. Morphological and chemical studies of collagen formation. I. The fine structure of guinea pig granulomata. J Biophys Biochem Cytol. 1961 Mar;9:639–651. doi: 10.1083/jcb.9.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLERMONT Y. The Golgi zone of the rat spermatid and its role in the formation of cytoplasmic vesicles. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):119–122. doi: 10.1083/jcb.2.4.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALTON A. J., FELIX M. D. Cytologic and cytochemical characteristics of the Golgi substance of epithelial cells of the epididymis in situ, in homogenates and after isolation. Am J Anat. 1954 Mar;94(2):171–207. doi: 10.1002/aja.1000940202. [DOI] [PubMed] [Google Scholar]
- FARQUHAR M. G., WELLINGS S. R. Electron microscopic evidence suggesting secretory granule formation within the Golgi apparatus. J Biophys Biochem Cytol. 1957 Mar 25;3(2):319–322. doi: 10.1083/jcb.3.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GATENBY J. B., DALTON A. J., FELIX M. D. The contractile vacuole of parazoa and protozoa, and the golgi apparatus. Nature. 1955 Aug 13;176(4476):301–302. doi: 10.1038/176301a0. [DOI] [PubMed] [Google Scholar]
- GODMAN G. C., PORTER K. R. Chondrogenesis, studied with the electron microscope. J Biophys Biochem Cytol. 1960 Dec;8:719–760. doi: 10.1083/jcb.8.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROSS J. The behavior of collagen units as a model in morphogenesis. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):261–274. doi: 10.1083/jcb.2.4.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACKSON D. S., BENTLEY J. P. On the significance of the extractable collagens. J Biophys Biochem Cytol. 1960 Feb;7:37–42. doi: 10.1083/jcb.7.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JAKUS M. A. Studies on the cornea. II. The fine structure of Descement's membrane. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):243–252. doi: 10.1083/jcb.2.4.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KARRER H. E. Electron microscope study of developing chick embryo aorta. J Ultrastruct Res. 1960 Dec;4:420–454. doi: 10.1016/s0022-5320(60)80032-9. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E. Intracisternal granules in the exocrine cells of the pancreas. J Biophys Biochem Cytol. 1956 Jul 25;2(4):417–422. doi: 10.1083/jcb.2.4.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALAY S. L., PALADE G. E. The fine structure of neurons. J Biophys Biochem Cytol. 1955 Jan;1(1):69–88. doi: 10.1083/jcb.1.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POTTER J. L., McCLUSKEY R. T., WEISSMANN G., THOMAS L. The removal of cartilage matrix by papain. Factors affecting the distribution of crystalline papain in vivo. J Exp Med. 1960 Dec 1;112:1173–1194. doi: 10.1084/jem.112.6.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REVEL J. P., NAPOLITANO L., FAWCETT D. W. Identification of glycogen in electron micrographs of thin tissue sections. J Biophys Biochem Cytol. 1960 Dec;8:575–589. doi: 10.1083/jcb.8.3.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSE G. G. The Golgi complex in living osteoblasts. J Biophys Biochem Cytol. 1961 Feb;9:463–478. doi: 10.1083/jcb.9.2.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHELDON H. An electron microscope study of the epithelium in the normal mature and immature mouse cornea. J Biophys Biochem Cytol. 1956 May 25;2(3):253–262. doi: 10.1083/jcb.2.3.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Biophys Biochem Cytol. 1960 Jul;7:619–630. doi: 10.1083/jcb.7.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. IV. Chemical and metabolic investigation of the ribonucleoprotein particles. J Biophys Biochem Cytol. 1959 Jan 25;5(1):1–10. doi: 10.1083/jcb.5.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SJOSTRAND F. S., HANZON V. Ultrastructure of Golgi apparatus of exocrine cells of mouse pancreas. Exp Cell Res. 1954 Nov;7(2):415–429. doi: 10.1016/s0014-4827(54)80087-5. [DOI] [PubMed] [Google Scholar]
- STRUNK S. W. The formation of intracellular crystals in midgut glands of Limnoria lignorum. J Biophys Biochem Cytol. 1959 May 25;5(3):385–392. doi: 10.1083/jcb.5.3.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THOMAS L. Reversible collapse of rabbit ears after intravenous papain, and prevention of recovery by cortisone. J Exp Med. 1956 Aug 1;104(2):245–252. doi: 10.1084/jem.104.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHALEY W. G., MOLLENHAUER H. H., KEPHART J. E. The endoplasmic reticulum and the Golgi structures in maize root cells. J Biophys Biochem Cytol. 1959 May 25;5(3):501–506. doi: 10.1083/jcb.5.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZELANDER T. Ultrastructure of articular cartilage. Z Zellforsch Mikrosk Anat. 1959;49(6):720–738. doi: 10.1007/BF00342718. [DOI] [PubMed] [Google Scholar]