Abstract
Colloidal gold particles, 25 to 55 A in diameter, which had been coated with polyvinylpyrrolidone, were microinjected into the ground cytoplasm of amebas (Chaos chaos). At time intervals of 1 minute, 2 minutes, 10 minutes, and 24 hours after injection the cells were fixed for electron microscopy. After 24 hours, gold particles were found in both the nuclei and the ground cytoplasm, the concentration being higher in the nuclei. Colloidal particles were also present in the nuclei after 10 minutes, but at this time interval the concentration did not appear to be greater than that in the ground cytoplasm. One and 2 minutes after injection, the gold particles were located almost exclusively in the ground cytoplasm; however, individual particles were often found within the annuli of the nuclear envelope, and were located specifically in the centers of these structures. The results suggest that at least some of the gold particles which enter the nuclei pass through the annuli, and that passage through these structures may be restricted to a central channel.
Full Text
The Full Text of this article is available as a PDF (740.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AFZELIUS B. A. The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope. Exp Cell Res. 1955 Feb;8(1):147–158. doi: 10.1016/0014-4827(55)90051-3. [DOI] [PubMed] [Google Scholar]
- FELDHERR C. M., MARSHALL J. M., Jr The use of colloidal gold for studies of intracellular exchanges in the ameba Chaos chaos. J Cell Biol. 1962 Mar;12:640–645. doi: 10.1083/jcb.12.3.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GAY H. Chromosome-nuclear membrane-cytoplasmic interrelations in Drosophila. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):407–414. [PMC free article] [PubMed] [Google Scholar]
- Goldstein L., Plaut W. DIRECT EVIDENCE FOR NUCLEAR SYNTHESIS OF CYTOPLASMIC RIBOSE NUCLEIC ACID. Proc Natl Acad Sci U S A. 1955 Nov 15;41(11):874–880. doi: 10.1073/pnas.41.11.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOORE R. D., MUMAW V. R., SCHOENBERG M. D. The transport and distribution of colloidal iron and its relation to the ultrastructure of the cell. J Ultrastruct Res. 1961 Jun;5:244–256. doi: 10.1016/s0022-5320(61)90018-1. [DOI] [PubMed] [Google Scholar]
- WATSON M. L. Further observations on the nuclear envelope of the animal cell. J Biophys Biochem Cytol. 1959 Oct;6:147–156. doi: 10.1083/jcb.6.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHALEY W. G., MOLLENHAUER H. H., LEECH J. H. Some observations on the nuclear envelope. J Biophys Biochem Cytol. 1960 Sep;8:233–245. doi: 10.1083/jcb.8.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WISCHNITZER S. An electron microscope study of the nuclear envelope of amphibian oocytes. J Ultrastruct Res. 1958 Apr;1(3):201–222. doi: 10.1016/s0022-5320(58)80001-5. [DOI] [PubMed] [Google Scholar]