Abstract
Immersion of the intestinal tissue, from rat jejunum, in hypertonic saline produced very rapid changes in all regions of the epithelial cells, but the apical region was apparently unaffected by hypotonic solutions for at least ½ hour. In both cases, blistering of the microvilli was taken as the first sign of degenerative changes which finally resulted in a breakdown to large vesicular particles. Consideration of both normal and modified tissue indicates that the core of the microvillus contains either paired strands or tubular structures. Lateral cross-fibres extended from the core to the microvillus membrane and may be an essential part of the supporting structure of the microvillus. Densitometer traces across the microvillus membrane at various stages of modification indicated that this membrane might include a 75 A unit membrane structure with additional components associated at either surface. Interruptions in the membrane were apparently expanded by the hypotonic solutions and these might possibly be distinguished from preparative artefacts.
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Selected References
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- CLARK S. L., Jr The ingestion of proteins and colloidal materials by columnar absorptive cells of the small intestine in suckling rats and mice. J Biophys Biochem Cytol. 1959 Jan 25;5(1):41–50. doi: 10.1083/jcb.5.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALTON A. J. Electron micrography of epithelial cells of the gastro-intestinal tract and pancreas. Am J Anat. 1951 Jul;89(1):109–133. doi: 10.1002/aja.1000890105. [DOI] [PubMed] [Google Scholar]
- GIBBONS I. R., GRIMSTONE A. V. On flagellar structure in certain flagellates. J Biophys Biochem Cytol. 1960 Jul;7:697–716. doi: 10.1083/jcb.7.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRANGER B., BAKER R. F. Electron microscope investigation of the striated border of intestinal epithelium. Anat Rec. 1950 Aug;107(4):423–441. doi: 10.1002/ar.1091070409. [DOI] [PubMed] [Google Scholar]
- KARRER H. E. Cell interconnections in normal human cervical epithelium. J Biophys Biochem Cytol. 1960 Feb;7:181–184. doi: 10.1083/jcb.7.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SCHWAB W. L'utilisation d'un copolymère du groupe des polyesters comme matériel d'inclusion en ultramicrotomie. Experientia. 1956 Nov 15;12(11):421–422. doi: 10.1007/BF02157363. [DOI] [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. I. The fasting animal. J Biophys Biochem Cytol. 1959 May 25;5(3):363–372. doi: 10.1083/jcb.5.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J Biophys Biochem Cytol. 1959 May 25;5(3):373–384. doi: 10.1083/jcb.5.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISS J. M. Mitochondrial changes induced by potassium and sodium in the duodenal absorptive cell as studied with the electron microscope. J Exp Med. 1955 Dec 1;102(6):783–788. doi: 10.1084/jem.102.6.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISS J. M. The role of the Golgi complex in fat absorption as studied with the electron microscope with observations on the cytology of duodenal absorptive cells. J Exp Med. 1955 Dec 1;102(6):775–782. doi: 10.1084/jem.102.6.775. [DOI] [PMC free article] [PubMed] [Google Scholar]