Abstract
A cell has been isolated from explanted rabbit liver which contains, during all phases of its growth in culture, hundreds of lipid-rich particles with a distinct limiting membrane. The cell grows logarithmically with a generation time of 19 to 20 hours and during mitosis the particles are distributed between the daughter cells. Associated with the particles is the high total lipid content of the rabbit liver cell as compared with a rat liver cell, which contains few, if any, lipid-rich particles. This difference in lipid content between the two cells is due primarily to an increase in the triglyceride fraction, in contradistinction to small differences in the polar lipid and sterol ester fractions. The lipid-rich particles have been isolated and found to contain 90 per cent triglyceride on a dry weight basis. The "genetic" factors responsible for the high concentration of lipid-rich particles and triglycerides in the rabbit liver cell require for their full expression one or more factors which are present in much higher effective concentrations in rabbit serum than in horse serum. The hypothesis is advanced that the lipid-rich particles represent a normal state of the non-structural cell lipid. A procedure is described for the quantitative isolation of the lipid of cultured cells.
Full Text
The Full Text of this article is available as a PDF (847.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
- BARRON E. J., HANAHAN D. J. Observations on the silicic acid chromatography of the neutral lipides of rat liver, beef liver, and yeast. J Biol Chem. 1958 Mar;231(1):493–503. [PubMed] [Google Scholar]
- EVANS V. J., BRYANT J. C., MCQUILKIN W. T., FIORAMONTI M. C., SANFORD K. K., WESTFALL B. B., EARLE W. R. Studies of nutrient media for tissue cells in vitro. II. An improved protein-free chemically defined medium for long-term cultivation of strain L-929 cells. Cancer Res. 1956 Jan;16(1):87–94. [PubMed] [Google Scholar]
- HARTROFT W. S. Pathology of lipid disorders: liver and cardiovascular system. Fed Proc. 1961 Mar;20(Suppl 7):135–145. [PubMed] [Google Scholar]
- KING D. W., SOCOLOW E. L., BENSCH K. G. The relation between protein synthesis and lipide accumulation in L strain cells and Ehrlich ascites cells. J Biophys Biochem Cytol. 1959 May 25;5(3):421–431. doi: 10.1083/jcb.5.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACKENZIE C. G., MACKENZIE J. B., BECK P. The effect of pH on growth, protein synthesis, and lipid-rich particles of cultured mammalian cells. J Biophys Biochem Cytol. 1961 Jan;9:141–156. doi: 10.1083/jcb.9.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARINETTI G. V., STOTZ E. Chromatography of phosphatides on silicic acid impregnated paper. Biochim Biophys Acta. 1956 Jul;21(1):168–170. doi: 10.1016/0006-3002(56)90108-1. [DOI] [PubMed] [Google Scholar]
- OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
- PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SANFORD K. K., EARLE W. R., LIKELY G. D. The growth in vitro of single isolated tissue cells. J Natl Cancer Inst. 1948 Dec;9(3):229–246. [PubMed] [Google Scholar]
- VAN HANDEL E., ZILVERSMIT D. B. Micromethod for the direct determination of serum triglycerides. J Lab Clin Med. 1957 Jul;50(1):152–157. [PubMed] [Google Scholar]
- WAGNER H., HOERHAMMER L., WOLFF P. [Thin layer chromatography of phosphatides and glycolipids]. Biochem Z. 1961;334:175–184. [PubMed] [Google Scholar]