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. 1962 Aug 1;14(2):183–192. doi: 10.1083/jcb.14.2.183

INCORPORATION OF TRITIATED CYTIDINE INTO RIBONUCLEIC ACID BY ISOLATED PEA NUCLEI

Joon H Rho 1, Margaret I Chipchase 1
PMCID: PMC2106106  PMID: 14491776

Abstract

A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H3 into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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