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. 1962 Dec 1;15(3):525–534. doi: 10.1083/jcb.15.3.525

CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

I. On the Particulate Nature of the DNA-Containing Elements

M Rabinovitch 1, W Plaut 1
PMCID: PMC2106172  PMID: 13972870

Abstract

The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detectable structures. Staining experiments with basic dyes, including the fluorochrome acridine orange, revealed the presence of large numbers of 0.3 to 0.5 µ particles in the cytoplasm of all cells studied. The effect of nuclease digestion on the dye affinity of the particles suggests that they contain DNA as well as RNA. Centrifugation of living cells at 10,000 g leads to the sedimentation of the particles in the centrifugal third of the ameba near the nucleus. Analysis of centrifuged cells which had been incubated with H3-thymidine showed a very high degree of correlation between the location of the nucleic acid-containing granules and that of acid-insoluble, deoxyribonuclease-sensitive labeled molecules and leads to the conclusion that cytoplasmic DNA synthesis in Amoeba proteus occurs in association with these particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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