Abstract
The effect of trypsin on the morphology of the rat liver microsomal fraction isolated by differential centrifugation has been investigated. The microsomes were incubated at 37°C and centrifuged thereafter under the conditions of their initial isolation. The trypsin-treated microsomes and the untreated controls were fixed in unbuffered osmium tetroxide and embedded first in gelose and then in methacrylate. In the trypsin-treated microsomes, there was a removal of the ribosomes from the rough vesicles. Parallel chemical determinations showed that the total nitrogen and total phosphorus of the pellet were lowered. Particles, densely stained with phosphotungstic acid (PTA) and homogeneous in appearance, were found within microsome smooth vesicles in a fluffy layer which collects on the top of the microsome pellet. The morphology of these PTA-stained particles remained unchanged after incubation with trypsin.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERNHARD W., HAGUENAU F., GAUTIER A., OBERLING C. La structure submicroscopique des elements basophiles cytoplasmiques dans le foie, le pancreas et les glandes salivaires; étude de coupes ultrafines au microscope électronique. Z Zellforsch Mikrosk Anat. 1952;37(3):281–300. [PubMed] [Google Scholar]
- Berenblum I., Chain E. An improved method for the colorimetric determination of phosphate. Biochem J. 1938 Feb;32(2):295–298. doi: 10.1042/bj0320295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHAUVEAU J., GAUTIER A., MOULE Y., ROUILLER C. Etude morphologique et biochimique de la fraction microsomes des cellules du foie et du pancréas de rat. C R Hebd Seances Acad Sci. 1955 Jul 18;241(3):337–339. [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DROCHMANS P. [Demonstration of glycogen in liver cells by electron microscopy]. J Biophys Biochem Cytol. 1960 Oct;8:553–558. [PMC free article] [PubMed] [Google Scholar]
- MOULE Y., ROUILLER C., CHAUVEAU J. A biochemical and morphological study of rat liver microsomes. J Biophys Biochem Cytol. 1960 Jun;7:547–558. doi: 10.1083/jcb.7.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markham R. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J. 1942 Dec;36(10-12):790–791. doi: 10.1042/bj0360790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E., PORTER K. R. Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954 Dec 1;100(6):641–656. doi: 10.1084/jem.100.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E., SIEKEVITZ P. Liver microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Mar 25;2(2):171–200. doi: 10.1083/jcb.2.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E., SIEKEVITZ P. Pancreatic microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Nov 25;2(6):671–690. doi: 10.1083/jcb.2.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PORTER K. R. Observations on a submicroscopic basophilic component of cytoplasm. J Exp Med. 1953 May;97(5):727–750. doi: 10.1084/jem.97.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROTH J. S. Studies on the function of intracellular ribonucleases. II. The interaction of ribonucleprotein and enzymes. J Biophys Biochem Cytol. 1960 Jun;7:443–453. doi: 10.1083/jcb.7.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHNEIDER W. C., HOGEBOOM G. H. Cytochemical studies of mammalian tissues; the isolation of cell components by differential centrifugation: a review. Cancer Res. 1951 Jan;11(1):1–22. [PubMed] [Google Scholar]
- SLAUTTERBACK D. B. Electron microscopic studies of small cytoplasmic particles (microsomes). Exp Cell Res. 1953 Sep;5(1):173–186. doi: 10.1016/0014-4827(53)90102-5. [DOI] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]