Abstract
Outgrowth of the stolon in the hydroid Cordylophora has been studied at the cellular level. Staining experiments and histological examination indicate that the generative region of the stolon is at its base where interstitial cells are prominent. Cells in the ectoderm at the stolon tip appear to be actively synthesizing new perisarc as the tip advances over the substrate, rather than involved in proliferation. The fine structure of cellular relationships in these regions has been considered and an attempt has been made to correlate the structure and distribution of intercellular attachments with these various functional zones along the outgrowing stolon. Intercellular attachments are similar to those previously described in Hydra, but favorable cases provide a more complete description. The series of intercellular septa each consist of two electron-opaque bodies lying close together midway between the two plasma membranes and separated from the membranes by a region of lesser density. The plasma membranes contain electron-opaque and transparent regions which alternate with the septa. These regions extend over about 70 A. The position of intercellular connections relative to the outer surface of the cenosarc varies in different parts of the stolon.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KUROSUMI K. Electron microscopic analysis of the secretion mechanism. Int Rev Cytol. 1961;11:1–124. doi: 10.1016/s0074-7696(08)62713-8. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ODLAND G. F. The fine structure of the interrelationship of cells in the human epidermis. J Biophys Biochem Cytol. 1958 Sep 25;4(5):529–538. [PMC free article] [PubMed] [Google Scholar]
- OVERTON J. Desmosome development in normal and reassociating cells in the early chick blastoderm. Dev Biol. 1962 Jun;4:532–548. doi: 10.1016/0012-1606(62)90056-8. [DOI] [PubMed] [Google Scholar]
- OVERTON J. Studies of the mode of outgrowth of the amphibian pronephric duct. J Embryol Exp Morphol. 1959 Mar;7(1):86–93. [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOOD R. L. Intercellular attachment in the epithelium of Hydra as revealed by electron microscopy. J Biophys Biochem Cytol. 1959 Dec;6:343–352. doi: 10.1083/jcb.6.3.343. [DOI] [PMC free article] [PubMed] [Google Scholar]