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. 1963 Apr 1;17(1):167–201. doi: 10.1083/jcb.17.1.167

A CORRELATED LIGHT AND ELECTRON MICROSCOPE STUDY OF THE NUCLEOLAR MATERIAL DURING MITOSIS IN VICIA FABA

J G Lafontaine 1, L A Chouinard 1
PMCID: PMC2106260  PMID: 13928037

Abstract

Root meristematic cells of Vicia faba were examined, with both light and electron microscopes, in order to study the behaviour of the nucleolar material during the mitotic process. Under light microscopy, the preprophase nucleolus is seen to consist of a densely stained material in which are embedded several unstained vacuole-like structures of varying size. The electron microscope reveals that the dense nucleolar material is formed of two structurally distinct components, each segregated into irregularly shaped zones blending with one another. One of these components is represented by 150 A granules which, in places, are arranged into thread-like structures approximately 0.1 µ in diameter; the other component apparently consists of fibrils 60 to 100 A in diameter. The large and medium sized intranucleolar vacuoles contain loosely scattered granules and fibrils similar to those just described. The granular and fibrillar components of the denser portion of the nucleolus persist as such during prophase and disperse throughout the nuclear cavity at the time of nucleolar disintegration. After nuclear membrane breakdown, these granules and fibrils, as well as those of the nucleoplasm, mix freely with similar elements already present within the forming spindle. No evidence has been obtained that, during or after nucleolar disintegration, the structural components of the nucleolus become associated as such with the chromosomes to form an external or internal matrix. Our observations suggest the existence, of a matrix substance within late prophase, metaphase, and anaphase chromosomes, the fine structure of which bears strong resemblance to that of their constituent coiled chromonemata. Data are presented, moreover, that indicate that part of this matrix substance, presumably formed at some time during prophase, is released from the chromosomes during their anaphasic movement. A number of observations indicate that the main bulk of the next nucleolus is derived from a prenucleolar fibrillogranular material, arranged into thread-like structures some 0.1 µ in diameter, which collect in the interchromosomal spaces during early and midtelophase. Finally, our data would seem to favour the view that most of this prenucleolar material results from a resumption of the synthetic activity of the early and midtelophase chromosomes rather than from a mere shedding of a preexisting matrix substance.

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Selected References

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  1. BERNHARD W., BAUER A., GROPP A., HAGUENAU F., OBERLING C. L'ultrastructure du nucléole de cellules normales et cancéreuses; étude au microscope électronique. Exp Cell Res. 1955 Aug;9(1):88–100. doi: 10.1016/0014-4827(55)90162-2. [DOI] [PubMed] [Google Scholar]
  2. BOSS J. Mitosis in cultures of newt tissues. IV. The cell surface in late anaphase and the movements of ribonucleoprotein. Exp Cell Res. 1955 Feb;8(1):181–187. doi: 10.1016/0014-4827(55)90055-0. [DOI] [PubMed] [Google Scholar]
  3. CHAYEN J., DAVIES H. G., MILES U. J. Observations on some plant interphase nuclei. Proc R Soc Lond B Biol Sci. 1953 Apr 17;141(903):190–199. doi: 10.1098/rspb.1953.0035. [DOI] [PubMed] [Google Scholar]
  4. DAS N. K. Demonstration of a non-RNA nucleolar fraction by silver staining. Exp Cell Res. 1962 Mar;26:428–431. doi: 10.1016/0014-4827(62)90195-7. [DOI] [PubMed] [Google Scholar]
  5. HORSTMANN E., KNOOP A. Zur Struktur des Nucleolus und des Kernes. Z Zellforsch Mikrosk Anat. 1957;46(1):100–107. [PubMed] [Google Scholar]
  6. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KAUFMANN B. P., GAY H., McDONALD M. R. Organizational patterns within chromosomes. Int Rev Cytol. 1960;9:77–127. doi: 10.1016/s0074-7696(08)62745-x. [DOI] [PubMed] [Google Scholar]
  8. KAUFMANN B. P., McDONALD M., GAY H. Enzymatic degradation of ribonucleoproteins of chromosomes, nucleoli and cytoplasm. Nature. 1948 Nov 20;162(4125):814–814. doi: 10.1038/162814a0. [DOI] [PubMed] [Google Scholar]
  9. LA COUR L. F., CHAYEN J. A cyclic staining behaviour of the chromosomes during mitosis and meiosis. Exp Cell Res. 1958 Jun;14(3):462–468. doi: 10.1016/0014-4827(58)90154-x. [DOI] [PubMed] [Google Scholar]
  10. LAFONTAINE J. G. Structure and mode of formation of the nucleolus in meristematic cells of Vicia faba and Allium cepa. J Biophys Biochem Cytol. 1958 Nov 25;4(6):777–784. doi: 10.1083/jcb.4.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LIN M. Chromosomal control of nuclear composition in maize. Chromosoma. 1955;7(4):340–370. doi: 10.1007/BF00329732. [DOI] [PubMed] [Google Scholar]
  12. PORTER K. R. Electron microscopy of basophilic components of cytoplasm. J Histochem Cytochem. 1954 Sep;2(5):346–375. doi: 10.1177/2.5.346. [DOI] [PubMed] [Google Scholar]
  13. PORTER K. R., MACHADO R. D. Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip. J Biophys Biochem Cytol. 1960 Feb;7:167–180. doi: 10.1083/jcb.7.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PRESCOTT D. M., BENDER M. A. Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. Exp Cell Res. 1962 Mar;26:260–268. doi: 10.1016/0014-4827(62)90176-3. [DOI] [PubMed] [Google Scholar]
  15. RIS H., KLEINFELD R. Cytochemical studies on the chromatin elimination of Solenobia (lepidopteria). Chromosoma. 1952;5(4):363–371. [PubMed] [Google Scholar]
  16. RUSTAD R. C. An interference microscopical and cytochemical analysis of local mass changes in the mitotic apparatus during mitosis. Exp Cell Res. 1959 Mar;16(3):575–583. doi: 10.1016/0014-4827(59)90125-9. [DOI] [PubMed] [Google Scholar]
  17. TANDLER C. J. The silver-reducing property of the nucleolus and the formation of prenucleolar material during mitosis. Exp Cell Res. 1959 Jun;17(3):560–564. doi: 10.1016/0014-4827(59)90087-4. [DOI] [PubMed] [Google Scholar]
  18. TAYLOR J. H. Nucleic acid synthesis in relation to the cell division cycle. Ann N Y Acad Sci. 1960 Oct 7;90:409–421. doi: 10.1111/j.1749-6632.1960.tb23259.x. [DOI] [PubMed] [Google Scholar]
  19. WOODARD J., RASCH E., SWIFT H. Nucleic acid and protein metabolism during the mitotic cycle in Vicia faba. J Biophys Biochem Cytol. 1961 Feb;9:445–462. doi: 10.1083/jcb.9.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]

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