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. 1963 Jul 1;18(1):13–17. doi: 10.1083/jcb.18.1.13

THE OCCURRENCE OF INTRACELLULAR CHONDROITIN SULFATE

Frank K Thorp 1, Albert Dorfman 1
PMCID: PMC2106285  PMID: 13981267

Abstract

Suspensions of chondrocytes were prepared by treatment with trypsin of the epiphyses of tibias and femurs of 13-day-old chick embryos. After washing to remove the matrix, such suspensions readily incorporate radioactive sulfate into both intracellular and extracellular chondroitin sulfate. Following disruption of the cells, the cell constituents were fractionated by centrifugation. Fractions obtained from cells incubated for 10 minutes showed a concentration of radioactivity in the material which sediments at 10,000 to 20,000 g. At this time the radioactivity of the extracellular chondroitin sulfate is low, but at 1 hour the radioactivity of the intracellular material is relatively unchanged, while that of the extracellular polysaccharide is markedly increased. Following incubation of the chondrocyte suspensions in a tissue culture medium, the intracellular chondroitin sulfate was isolated. This was compared with chondroitin sulfate isolated from the cartilage matrix. Chemical analysis and infrared spectroscopy indicated that both the intracellular and extracellular polysaccharides consist of a mixture of chondroitin sulfuric acids A and C. A portion of the chondroitin sulfate is not sulfated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS J. B. The biosynthesis of chondroitin sulphate. Incorporation of sulphate into chondroitin sulphate in embryonic chick cartilage. Biochem J. 1960 Sep;76:520–533. doi: 10.1042/bj0760520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BELANGER L. F. Autoradiographic visualization of the entry and transit of S35 in cartilage, bone, and dentine of young rats and the effect of hyaluronidase in vitro. Can J Biochem Physiol. 1954 May;32(3):161–169. [PubMed] [Google Scholar]
  3. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  4. CAMPO R. D., DZIEWIATKOWSKI D. D. Intracellular synthesis of protein-polysaccharides by slices of bovine costal cartilage. J Biol Chem. 1962 Sep;237:2729–2735. [PubMed] [Google Scholar]
  5. D'ABRAMO F., LIPMANN F. The formation of adenosine-3'-phosphate-5'-phosphosulfate in extracts of chick embryo cartilage and its conversion into chondroitin sulfate. Biochim Biophys Acta. 1957 Jul;25(1):211–213. doi: 10.1016/0006-3002(57)90452-3. [DOI] [PubMed] [Google Scholar]
  6. DZIEWIATKOWSKI D. D. Intracellular synthesis of chondroitin sulfate. J Cell Biol. 1962 Jun;13:359–364. doi: 10.1083/jcb.13.3.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GODMAN G. C., PORTER K. R. Chondrogenesis, studied with the electron microscope. J Biophys Biochem Cytol. 1960 Dec;8:719–760. doi: 10.1083/jcb.8.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GROSS J. I., MATHEWS M. B., DORFMAN A. Sodium chondroitin sulfate-protein complexes of cartilage. II. Metabolism. J Biol Chem. 1960 Oct;235:2889–2892. [PubMed] [Google Scholar]
  9. MARKOVITZ A., CIFONELLI J. A., DORFMAN A. The biosynthesis of hyaluronic acid by group A Streptococcus. VI. Biosynthesis from uridine nucleotides in cell-free extracts. J Biol Chem. 1959 Sep;234:2343–2350. [PubMed] [Google Scholar]
  10. MARKOVITZ A., DORFMAN A. Synthesis of capsular polysaccharide (hyaluronic acid) by protoplastmembrane preparations of group A Streptococcus. J Biol Chem. 1962 Feb;237:273–279. [PubMed] [Google Scholar]
  11. MATHEWS M. B., INOUYE M. The determination of chondroitin sulfate C-type polysaccharides in mixtures with other acid mucopolysaccharides. Biochim Biophys Acta. 1961 Nov 11;53:509–513. doi: 10.1016/0006-3002(61)90209-8. [DOI] [PubMed] [Google Scholar]
  12. MATHEWS M. B., ROSEMAN S., DORFMAN A. Determination of the chondroitinase activity of bovine testicular preparations. J Biol Chem. 1951 Jan;188(1):327–334. [PubMed] [Google Scholar]
  13. MOSCONA A., MOSCONA H. The dissociation and aggregation of cells from organ rudiments of the early chick embryo. J Anat. 1952 Jul;86(3):287–301. [PMC free article] [PubMed] [Google Scholar]
  14. MUIR H. The nature of the link between protein and carbohydrate of a chondroitin sulphate complex from hyaline cartilage. Biochem J. 1958 Jun;69(2):195–204. doi: 10.1042/bj0690195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. OKADA T. S., SIRLIN J. L. The distribution of sulphur in the differentiating visceral cartilage of Xenopus. J Embryol Exp Morphol. 1960 Mar;8:54–59. [PubMed] [Google Scholar]
  16. PELC S. R., GLUCKSMANN A. Sulphate metabolism in the cartilage of the trachea, pinna and xiphoid process of the adult mouse as indicated by autoradiographs. Exp Cell Res. 1955 Apr;8(2):336–344. doi: 10.1016/0014-4827(55)90145-2. [DOI] [PubMed] [Google Scholar]
  17. SUZUKI S., STROMINGER J. L. Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides. J Biol Chem. 1960 Feb;235:257–266. [PubMed] [Google Scholar]
  18. SUZUKI S., STROMINGER J. L. Enzymatic sulfation of mucopolysaccharides in hen oviduct. II. Mechanism of the reaction studied with oligosaccharides and monosaccharides as acceptors. J Biol Chem. 1960 Feb;235:267–273. [PubMed] [Google Scholar]

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