Abstract
Portions of mink endometrium in delayed implantation, early postimplantation, and pseudo pregnancy were fixed in buffered osmium tetroxide with sucrose, or potassium permanganate. After rapid dehydration the portions of endometrium were embedded in either methacrylate or epoxy resin. Examination of the cells from the body of the glands of the endometrium of delayed implantation revealed the presence of prominent terminal bars, numerous secretion granules, and membrane discs in the apical region of the cell. In the supranuclear and infranuclear regions, mildly dilated cisternae of the endoplasmic reticulum were present, and in many cells unusually large mitochondria were seen. Numerous changes were noted in the gland cells of the post implantation stage. The endoplasmic reticulum in the basal region was extensively dilated, and the nuclei were situated more centrally. Giant mitochondria were no longer present. The large secretion granules were not present, but smaller granules were seen, especially in the Golgi region. Some of the Golgi cisternae were dilated and the pattern of parallel membranes was consequently less distinct. It is suggested that gland cells in the postimplantation and pseudopregnancy stages exhibit evidence of greater secretory activity than those in the delayed implantation stage.
Full Text
The Full Text of this article is available as a PDF (1.5 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARNES B. G. Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinology. 1962 Oct;71:618–628. doi: 10.1210/endo-71-4-618. [DOI] [PubMed] [Google Scholar]
- BRANDES D., GROTH D. P., GYORKEY F. Occurrence of lysosomes in the prostatic epithelium of castrate rats. Exp Cell Res. 1962 Oct;28:61–68. doi: 10.1016/0014-4827(62)90312-9. [DOI] [PubMed] [Google Scholar]
- BRANDES D., PORTELA A. The fine structure of the epithelial cells of the mouse prostate. I. Coagulating gland epithelium. J Biophys Biochem Cytol. 1960 Jun;7:505–510. doi: 10.1083/jcb.7.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALTON A. J., ZEIGEL R. F. A simplified method of staining thin sections of biolgical material with lead hydroxide for electron microscopy. J Biophys Biochem Cytol. 1960 Apr;7:409–410. doi: 10.1083/jcb.7.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEANE H. W., PORTER K. R. A comparative study of cytoplasmic basophilia and the population density of ribosomes in the secretory cells of mouse seminal vesicle. Z Zellforsch Mikrosk Anat. 1960;52:697–711. doi: 10.1007/BF00336621. [DOI] [PubMed] [Google Scholar]
- DEMPSEY E. W., WISLOCKI G. B., AMOROSO E. C. Electron microscopy of the pig's placenta, with especial reference to the cell membranes of the endometrium and chorion. Am J Anat. 1955 Jan;96(1):65–101. doi: 10.1002/aja.1000960104. [DOI] [PubMed] [Google Scholar]
- DEMPSEY E. W., WISLOCKI G. B. Electron microscopic observations on the placenta of the cat. J Biophys Biochem Cytol. 1956 Nov 25;2(6):743–754. doi: 10.1083/jcb.2.6.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ENDERS A. C. Electron microscopic observations on the villous haemochorial placenta of the nine-banded armadillo (Dasypus novemcinctus). J Anat. 1960 Apr;94:205–215. [PMC free article] [PubMed] [Google Scholar]
- ENDERS A. C. Histological observations on the chorio-allantoic placenta of the mink. Anat Rec. 1957 Feb;127(2):231–245. doi: 10.1002/ar.1091270209. [DOI] [PubMed] [Google Scholar]
- FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FARQUHAR M. G., RINEHART J. F. Electron microscopic studies of the anterior pituitary gland of castrate rats. Endocrinology. 1954 May;54(5):516–541. doi: 10.1210/endo-54-5-516. [DOI] [PubMed] [Google Scholar]
- FAWCETT D. W., SELBY C. C. Observations on the fine structure of the turtle atrium. J Biophys Biochem Cytol. 1958 Jan 25;4(1):63–72. doi: 10.1083/jcb.4.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LARSEN J. F. Electron microscopy of the uterine epithelium in the rabbit. J Cell Biol. 1962 Jul;14:49–64. doi: 10.1083/jcb.14.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NILSSON O. Correlation of structure to function of the luminal cell surface in the uterine epithelium of mouse and man. Z Zellforsch Mikrosk Anat. 1962;56:803–808. doi: 10.1007/BF00336335. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Electron microscopy of the glandular epithelium in the human uterus. I. Follicular phase. J Ultrastruct Res. 1962 May;6:413–421. doi: 10.1016/s0022-5320(62)80044-6. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Electron microscopy of the glandular epithelium in the human uterus. II. Early and late luteal phase. J Ultrastruct Res. 1962 May;6:422–431. doi: 10.1016/s0022-5320(62)80045-8. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Ultrastructure of mouse uterine surface epithelium under different estrogenic influence. 4. Uterine secretion. J Ultrastruct Res. 1959 Mar;2(3):331–341. doi: 10.1016/s0022-5320(59)80005-8. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Ultrastructure of mouse uterine surface epithelium under different estrogenic influences. 1. Spayed animals and oestrous animals. J Ultrastruct Res. 1958 Aug;1(4):375–396. doi: 10.1016/s0022-5320(58)90009-1. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Ultrastructure of mouse uterine surface epithelium under different estrogenic influences. 2. Early effect of estrogen administered to spayed animals. J Ultrastruct Res. 1958 Nov;2(1):73–95. doi: 10.1016/s0022-5320(58)90048-0. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Ultrastructure of mouse uterine surface epithelium under different estrogenic influences. 3. Late effect of estrogen administered to spayed animals. J Ultrastruct Res. 1958 Dec;2(2):185–199. doi: 10.1016/s0022-5320(58)90017-0. [DOI] [PubMed] [Google Scholar]
- NILSSON O. Ultrastructure of mouse uterine surface epithelium under different estrogenic influences. 5. Continuous administration of estrogen. J Ultrastruct Res. 1959 Mar;2(3):342–351. doi: 10.1016/s0022-5320(59)80006-x. [DOI] [PubMed] [Google Scholar]
- WISLOCKI G. B., WEISS L. P., BURGOS M. H., ELLIS R. A. The cytology, histochemistry and electron microscopy of the granular cells of the metrial gland of the gravid rat. J Anat. 1957 Jan;91(1):130–140. [PMC free article] [PubMed] [Google Scholar]
- ZEIGEL R. F., DALTON A. J. Speculations based on the morphology of the Golgi systems in several types of proteinsecreting cells. J Cell Biol. 1962 Oct;15:45–54. doi: 10.1083/jcb.15.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]