Abstract
It has been demonstrated that the nature of the physical change in mitochondrial membrane fragments associated with the action of the respiratory enzymes is likely one of shape or symmetry rather than size. The findings suggest that in the state of decreased scattering the macromolecules may be present in an extended physical state. Conditions favorable for phosphorylation may give rise to a folding or contraction of the molecular complex to a more symmetrical structure. Since earlier studies have shown that there is a compulsory relationship between the integrity of systems operative in oxidative phosphorylation and scattering changes, experiments of this type may lead to values for the minimal size of a phosphorylating unit, which at present is estimated to be 2.1 x 106 from light-scattering studies.
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Selected References
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- BLUM J. J., MORALES M. F. The interaction of myosin with adenosine triphosphate. Arch Biochem Biophys. 1953 Mar;43(1):208–230. doi: 10.1016/0003-9861(53)90100-3. [DOI] [PubMed] [Google Scholar]
- DEVLIN T. M., LEHNINGER A. L. The preparation of phosphorylating subfragments of rat liver mitochondria with digitonin. J Biol Chem. 1958 Dec;233(6):1586–1588. [PubMed] [Google Scholar]
- GREEN D. E., ODA T. On the unit of mitochondrial structure and function. J Biochem. 1961 Jun;49:742–757. doi: 10.1093/oxfordjournals.jbchem.a127366. [DOI] [PubMed] [Google Scholar]
- JACKSON K. L., PACE N. Some permeability properties of isolated rat liver cell mitochondria. J Gen Physiol. 1956 Sep 20;40(1):47–71. doi: 10.1085/jgp.40.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEHNINGER A. L. Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol Rev. 1962 Jul;42:467–517. doi: 10.1152/physrev.1962.42.3.467. [DOI] [PubMed] [Google Scholar]
- OHNISHI T., OHNISHI T. Extraction of actin- and myosin-like proteins from liver mitochondria. J Biochem. 1962 Sep;52:230–231. doi: 10.1093/oxfordjournals.jbchem.a127604. [DOI] [PubMed] [Google Scholar]
- PACKER L. Metabolic and structural states of mitochondria. I. Regulation by adenosine diphosphate. J Biol Chem. 1960 Jan;235:242–249. [PubMed] [Google Scholar]
- PACKER L. Metabolic and structural states of mitochondria. II. Regulation by phosphate. J Biol Chem. 1961 Jan;236:214–220. [PubMed] [Google Scholar]
- PACKER L., TAPPEL A. L. Light scattering changes linked to oxidative phosphorylation in mitochondrial membrane fragments. J Biol Chem. 1960 Feb;235:525–530. [PubMed] [Google Scholar]
- SIEKEVITZ P., WATSON M. L. Some cytochemical characteristics of a phosphorylating digitonin preparation of mitochondria. Biochim Biophys Acta. 1957 Aug;25(2):274–279. doi: 10.1016/0006-3002(57)90469-9. [DOI] [PubMed] [Google Scholar]