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. 1963 Dec 1;19(3):467–475. doi: 10.1083/jcb.19.3.467

THE CYTONUCLEOPROTEINS OF AMEBAE

II. Some Aspects of Cytonucleoprotein Behavior and Synthesis

Thomas J Byers 1, Dorothy B Platt 1, Lester Goldstein 1
PMCID: PMC2106339  PMID: 14086132

Abstract

Radioactivity, apparently in cytonucleoproteins, from an amino acid-labeled nucleus implanted into a non-radioactive cell appeared in the host nucleus within 10 minutes, and the typical equilibrium ratio 70:30 donor nucleus radioactivity:host nucleus radioactivity was reached in 4 to 5 hours at 25°C. If such binucleates grew and divided, no localization of radioactivity was observable in cells fixed during mitosis, but the protein label remained concentrated in the daughter interphase nuclei for at least 4 generations. Continued migration of cytonucleoproteins was observed if these daughter nuclei were transplanted to other unlabeled cells. The Q 10 (19° to 29°C) of the migration rate of radioactive cytonucleoproteins was ca. 1.3, suggesting that passage through the cytoplasm occurred by diffusion. Both non-migratory nuclear proteins and cytonucleoproteins appear to be synthesized in the cytoplasm.

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Selected References

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