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. 1964 Jan 1;20(1):85–94. doi: 10.1083/jcb.20.1.85

A STUDY OF THE FINE STRUCTURE OF THE EPIDERMIS OF RANA PIPIENS

Paul F Parakkal 1, A Gedeon Matoltsy 1
PMCID: PMC2106343  PMID: 14105219

Abstract

The epidermis of adult Rana pipiens has been studied by electron microscopy and histological and histochemical methods. It was found that the epidermis is engaged in the production of both keratin and mucus. The basal cells are mainly filled with tonofilaments, whereas the cells located in the mid-portion of the epidermis contain both tonofilaments and mucous granules. Golgi vesicles and endoplasmic reticulum are found in relative abundance in the mucus-producing cells and seem to be involved in the production of mucous granules. The mucus seen was partly retained within the cells and partly secreted into the intercellular spaces. The outermost keratinized cells contain mainly filaments and a few remnants of cell constituents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRODY I. An ultrastructural study on the role of the keratohyalin granules in the keratinization process. J Ultrastruct Res. 1959 Oct;3:84–104. doi: 10.1016/s0022-5320(59)80018-6. [DOI] [PubMed] [Google Scholar]
  2. CHAPMAN G. B., DAWSON A. B. Fine structure of the larval anuran epidermis, with special reference to the figures of Eberth. J Biophys Biochem Cytol. 1961 Jul;10:425–435. doi: 10.1083/jcb.10.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHARLES A. An electron microscope study of cornification in the human skin. J Invest Dermatol. 1959 Aug;33:65–74. doi: 10.1038/jid.1959.123. [DOI] [PubMed] [Google Scholar]
  4. CHARLES A., SMIDDY F. G. The tonofibrils of the human epidermis. J Invest Dermatol. 1957 Nov;29(5):327–338. doi: 10.1038/jid.1957.108. [DOI] [PubMed] [Google Scholar]
  5. CLARK W. H., Jr, HIBBS R. G. Electron microscope studies of the human epidermis: the clear cell of Masson (dendritic cell or melanocyte). J Biophys Biochem Cytol. 1958 Nov 25;4(6):679–684. doi: 10.1083/jcb.4.6.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FELL H. B., MELLANBY E. Metaplasia produced in cultures of chick ectoderm by high vitamin A. J Physiol. 1953 Mar;119(4):470–488. doi: 10.1113/jphysiol.1953.sp004860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FELL H. B. The effect of excess vitamin A on cultures of embryonic chicken skin explanted at different stages of differentiation. Proc R Soc Lond B Biol Sci. 1956 Mar 26;146(923):242–256. doi: 10.1098/rspb.1957.0008. [DOI] [PubMed] [Google Scholar]
  8. HIBBS R. G., CLARK W. H., Jr Electron microscope studies of the human epidermis; the cell boundaries and topography of the stratum malpighii. J Biophys Biochem Cytol. 1959 Aug;6(1):71–76. doi: 10.1083/jcb.6.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HORSTMANN E., KNOOP A. Elektronenmikroskopische Studien an der Epidermis. I. Rattenpfote. Z Zellforsch Mikrosk Anat. 1958;47(3):348–362. [PubMed] [Google Scholar]
  10. LEESON C. R., THREADGOLD L. T. The differentiation of the epidermis in Rana pipiens. Acta Anat (Basel) 1961;44:159–173. doi: 10.1159/000141717. [DOI] [PubMed] [Google Scholar]
  11. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MATOLTSY A. G., MATOLTSY M. A study of morphological and chemical properties of keratohyalin granules. J Invest Dermatol. 1962 Apr;38:237–247. [PubMed] [Google Scholar]
  13. ODLAND G. F. The fine structure of the interrelationship of cells in the human epidermis. J Biophys Biochem Cytol. 1958 Sep 25;4(5):529–538. [PMC free article] [PubMed] [Google Scholar]
  14. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SELBY C. C. An electron microscope study of the epidermis of mammalian skin in thin sections. I. Dermo-epidermal junction and basal cell layer. J Biophys Biochem Cytol. 1955 Sep 25;1(5):429–444. doi: 10.1083/jcb.1.5.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SELBY C. C. An electron microscope study of thin sections of human skin. II. Superficial cell layers of footpad epidermis. J Invest Dermatol. 1957 Aug;29(2):131–149. doi: 10.1038/jid.1957.80. [DOI] [PubMed] [Google Scholar]
  17. WEISS P., FERRIS W. Electronmicrograms of larval amphibian epidermis. Exp Cell Res. 1954 May;6(2):546–549. doi: 10.1016/0014-4827(54)90210-4. [DOI] [PubMed] [Google Scholar]
  18. WEISS P., JAMES R. Skin metaplasia in vitro induced by brief exposure to vitamin A. Exp Cell Res. 1955;(Suppl 3):381–394. [PubMed] [Google Scholar]
  19. ZELICKSON A. S., HARTMANN J. F. An electron icroscopic study of human epidermis. J Invest Dermatol. 1961 Feb;36:65–72. [PubMed] [Google Scholar]
  20. ZELICKSON A. S. Normal human keratinization processes as demonstrated by electron microscopy. J Invest Dermatol. 1961 Nov;37:369–379. [PubMed] [Google Scholar]
  21. von BARGEN [Electron microscopic study of rabbit skin (epidermis)]. Z Zellforsch Mikrosk Anat. 1959;50:459–471. [PubMed] [Google Scholar]

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