Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1964 Jan 1;20(1):95–111. doi: 10.1083/jcb.20.1.95

ELECTRON MICROSCOPIC OBSERVATIONS ON THE ACCUMULATION OF DIVALENT CATIONS IN INTRAMITOCHONDRIAL GRANULES

Lee D Peachey 1
PMCID: PMC2106346  PMID: 14105220

Abstract

Electron microscopic evidence is presented, from mitochondria in whole cells of toad urinary bladder and from isolated rat kidney mitochondria, indicating that the divalent cations calcium, strontium, and barium are accumulated in granules localized in the mitochondrial matrix. This accumulation occurs under conditions in which divalent ions are present in the medium bathing either whole cells or isolated mitochondria. The evidence indicates that the divalent ions are deposited on, or in a pre-existing granule, possibly in exchange for other ions. It suggests a possible role of the intramitochondrial granules in the regulation of the internal ionic environment of the mitochondrion. Certain biochemical and physiological implications of this phenomenon are discussed.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOORE J. E., BARTLEY W. The permeability of isolated rat-liver mitochondria to sucrose, sodium chloride and potassium chloride at 0 degrees. Biochem J. 1958 Jun;69(2):223–236. doi: 10.1042/bj0690223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTLEY W., AMOORE J. E. The effects of manganese on the solute content of rat-liver mitochondria. Biochem J. 1958 Jul;69(3):348–360. doi: 10.1042/bj0690348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BENTLEY P. J. The effects of neurohypophysial extracts on the water transfer across the wall of the isolated urinary bladder of the toad Bufo marinus. J Endocrinol. 1958 Sep;17(3):201–209. doi: 10.1677/joe.0.0170201. [DOI] [PubMed] [Google Scholar]
  4. BRIERLEY G. P., BACHMANN E., GREEN D. E. Active transport of inorganic phosphate and magnesium ions by beef heart mitochondria. Proc Natl Acad Sci U S A. 1962 Nov 15;48:1928–1935. doi: 10.1073/pnas.48.11.1928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DELUCA H. F., ENGSTROM G. W. Calcium uptake by rat kidney mitochondria. Proc Natl Acad Sci U S A. 1961 Nov 15;47:1744–1750. doi: 10.1073/pnas.47.11.1744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DELUCA H. F., ENGSTROM G. W., RASMUSSEN H. The action of vitamin D and parathyroid hormone in vitro on calcium uptake and release by kidney mitochondria. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1604–1609. doi: 10.1073/pnas.48.9.1604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DELUCA H. F., REISER S., STEENBOCK H., KAESBERG P. Vitamin D and the structure of kidney mitochondria. Biochim Biophys Acta. 1960 Jun 3;40:526–530. doi: 10.1016/0006-3002(60)91394-9. [DOI] [PubMed] [Google Scholar]
  8. ENGSTROM G. W., DELUCA H. F. The action of vitamin D in vivo and in vitro on the release of calcium from kidney mitochondria. J Biol Chem. 1962 Mar;237:PC974–PC975. [PubMed] [Google Scholar]
  9. HUNTER F. E., Jr, FORD L. Inactivation of oxidative and phosphorylative systems in mitochondria by preincubation with phosphate and other ions. J Biol Chem. 1955 Sep;216(1):357–369. [PubMed] [Google Scholar]
  10. LEHNINGER A. L., ROSSI C. S., GREENAWALT J. W. Respiration-dependent accumulation of inorganic phosphate and Ca ions by rat liver mitochondria. Biochem Biophys Res Commun. 1963 Mar 25;10:444–448. doi: 10.1016/0006-291x(63)90377-2. [DOI] [PubMed] [Google Scholar]
  11. MAYNARD L. S., COTZIAS G. C. The partition of manganese among organs and intracellular organelles of the rat. J Biol Chem. 1955 May;214(1):489–495. [PubMed] [Google Scholar]
  12. MRAZ F. R. Calcium and strontium uptake by rat liver and kidney mitochondria. Proc Soc Exp Biol Med. 1962 Nov;111:429–431. doi: 10.3181/00379727-111-27814. [DOI] [PubMed] [Google Scholar]
  13. PEACHEY L. D., RASMUSSEN H. Structure of the toad's urinary bladder as related to its physiology. J Biophys Biochem Cytol. 1961 Aug;10:529–553. doi: 10.1083/jcb.10.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. POCHE R. [Electron microscopic studies on the morphology of the myocardium of the dormouse during the active and the lethargic state]. Z Zellforsch Mikrosk Anat. 1959;50:332–360. [PubMed] [Google Scholar]
  15. SLATER E. C., CLELAND K. W. Stabilization of oxidative phosphorylation in heart-muscle sarcosomes. Nature. 1952 Jul 19;170(4316):118–119. doi: 10.1038/170118b0. [DOI] [PubMed] [Google Scholar]
  16. SLATER E. C., CLELAND K. W. The effect of calcium on the respiratory and phosphorylative activities of heart-muscle sarcosomes. Biochem J. 1953 Nov;55(4):566–590. doi: 10.1042/bj0550566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TAPLEY D. F. The effect of thyroxine and other substances on the swelling of isolated rat liver mitochondria. J Biol Chem. 1956 Sep;222(1):325–339. [PubMed] [Google Scholar]
  18. THIERS R. E., REYNOLDS E. S., VALLEE B. L. The effect of carbon tetrachloride poisoning on subcellular metal distribution in rat liver. J Biol Chem. 1960 Jul;235:2130–2133. [PubMed] [Google Scholar]
  19. VASINGTON F. D., MURPHY J. V. Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem. 1962 Aug;237:2670–2677. [PubMed] [Google Scholar]
  20. WEISS J. M. Mitochondrial changes induced by potassium and sodium in the duodenal absorptive cell as studied with the electron microscope. J Exp Med. 1955 Dec 1;102(6):783–788. doi: 10.1084/jem.102.6.783. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES